J.J., Luszczki Supplementary Material for: Isobolographic Analysis of Interaction for Three-Drug Combination of Carbamazepine, Phenobarbital and Topiramate in the Mouse Maximal Electroshock-Induced Seizure Model <b><i>Background/Aims:</i></b> To characterize the anticonvulsant effects of a combination of 3 antiepileptic drugs (AEDs; i.e. carbamazepine (CBZ), phenobarbital (PB) and topiramate (TPM)) at the fixed-ratio of 1:1:1 in the mouse maximal electroshock (MES)-induced seizure model. <b><i>Methods:</i></b> Tonic hind limb extension (seizure activity) was evoked in adult male albino Swiss mice by a current (sine-wave, 25 mA, 500 V, 50 Hz, 0.2 s stimulus duration) delivered via auricular electrodes. Type I isobolographic analysis for parallel dose-response relationship curves (DRRCs) was used to analyze the 3-drug combination. <b><i>Results:</i></b> In the mouse MES model, all the studied AEDs (i.e. CBZ, PB and TPM) administered singly had their DRRCs parallel to each other. With type I isobolography for parallel DRRCs, a combination of CBZ, PB and TPM at the fixed-ratio of 1:1:1 exerted supra-additive (synergistic) interaction in the mouse MES model. <b><i>Conclusions:</i></b> Synergistic interaction among CBZ, PB and TPM at the fixed-ratio of 1:1:1 against MES-induced seizures is worthy of consideration in further clinical settings. All detailed calculations required to perform type I isobolographic analysis for the 3-drug combination were presented. Antiepileptic drugs;Isobolographic analysis;Maximal electroshock;Three-drug combination 2016-02-25
    https://karger.figshare.com/articles/dataset/Supplementary_Material_for_Isobolographic_Analysis_of_Interaction_for_Three-Drug_Combination_of_Carbamazepine_Phenobarbital_and_Topiramate_in_the_Mouse_Maximal_Electroshock-Induced_Seizure_Model/5129584
10.6084/m9.figshare.5129584.v1