10.6084/m9.figshare.5128663.v1 Athanasiadis D. Athanasiadis D. Kapelouzou A. Kapelouzou A. Martikos G. Martikos G. Katsimpoulas M. Katsimpoulas M. Schizas D. Schizas D. Vasdekis S.N. Vasdekis S.N. Kostakis A. Kostakis A. Liakakos T.D. Liakakos T.D. Lazaris A.M. Lazaris A.M. Supplementary Material for: Remote Ischemic Preconditioning May Attenuate Renal Ischemia-Reperfusion Injury in a Porcine Model of Supraceliac Aortic Cross-Clamping Karger Publishers 2015 Remote preconditioning Renal ischemia Suprarenal clamping 2015-10-20 00:00:00 Dataset https://karger.figshare.com/articles/dataset/Supplementary_Material_for_Remote_Ischemic_Preconditioning_May_Attenuate_Renal_Ischemia-Reperfusion_Injury_in_a_Porcine_Model_of_Supraceliac_Aortic_Cross-Clamping/5128663 <b><i>Aim:</i></b> The effect of remote ischemic preconditioning (RIPC) in decreasing renal ischemia-reperfusion injury (IRI) during a suprarenal aortic cross-clamping was examined in a swine model. <b><i>Materials and Methods:</i></b> Four groups of pigs were examined: (a) ischemia-reperfusion (IR) group, renal IRI produced by 30 min of supraceliac aortic cross-clamping; (b) RIPC I group, the same renal IRI following RIPC by brief occlusion of the infrarenal aorta (15 min ischemia and 15 min reperfusion); (c) RIPC II group, the same renal IRI following RIPC by brief occlusion of the infrarenal aorta (3 cycles of 5 min ischemia and 5 min reperfusion); (d) sham group. Renal function was assessed before and after IRI by examining creatinine, neutrophil gelatinase-associated lipocalin (NGAL), TNF-α, malondialdehyde (MDA), cystatin C and C-reactive protein (CRP) from renal vein blood samples at specific time intervals. <b><i>Results:</i></b> Both RIPC groups presented significantly less impaired results compared to the IR group when considering MDA, cystatin C, CRP and creatinine. Between the two RIPC groups, RIPC II presented a better response with regard to CRP, NGAL, TNF-α, MDA and cystatin C. <b><i>Conclusions:</i></b> Remote IR protocols and mainly repetitive short periods of cycles of IR ameliorate the biochemical kidney effects of IRI in a model of suprarenal aortic aneurysm repair.