Supplementary Material for: Involvement of the L-Type Amino Acid Transporter Lat2 in the Transport of 3,3′-Diiodothyronine across the Plasma Membrane A.Kinne M.Wittner E.K.Wirth K.M.Hinz R.Schülein J.Köhrle G.Krause 2015 Thyroid hormones are transported across cell membranes by transmembrane transporter proteins, for example by members of the monocarboxylate transporter (MCT) and the L-type amino acid transporter (LAT) families. LATs consist of a light chain (e.g. LAT2) and a heavy chain (CD98), which is essential for their cell surface expression and functionality. The specificity of Lat2 for thyroid hormones and their metabolites and its role in their transport was not fully clear. This fact motivated us to establish a cell system to elucidate the uptake of thyroid hormones and their metabolites by mouse Lat2. The coinjection of cRNA coding for Lat2 and CD98 into <i>Xenopus laevis</i> oocytes resulted in a markedly increased level of 3,3′-diiodo-L-thyronine (3,3′-T<sub>2</sub>) and to some extent also enhanced T<sub>3</sub> transport. To gain insight into properties of thyroid hormones and their metabolites transported by Lat2, we inhibited 3,3′-T<sub>2</sub> uptake by various iodothyronine derivatives. T<sub>1</sub> and T<sub>2</sub> derivatives as well as 2-aminobicyclo-[2,2,1]-heptane-2-carboxylic acid strongly competed with 3,3′-T<sub>2</sub> uptake. In addition, we performed T<sub>2</sub> uptake measurements with the thyroid hormone-specific transporter MCT8. For both Lat2 and MCT8, K<sub>m</sub> values in a low micromolar range were calculated. We demonstrated that oocytes are a suitable system for thyroid hormone transport studies mediated by Lat2. Our data indicates that Lat2 compared to other thyroid hormone transporters prefers 3,3′-T<sub>2</sub> as the substrate. Thus, Lat2 might contribute to the availability of thyroid hormone by importing and/or exporting 3,3′-T<sub>2</sub>, which is generated either by T<sub>3</sub> inactivation or by rapid deiodinase 1-mediated rT<sub>3</sub> degradation.