%0 Generic %A H., Saadi %A D.-G.I., Reteno %A P., Colson %A S., Aherfi %A P., Minodier %A I., Pagnier %A D., Raoult %A La Scola B. %D 2013 %T Supplementary Material for: Shan Virus: A New Mimivirus Isolated from the Stool of a Tunisian Patient with Pneumonia %U https://karger.figshare.com/articles/dataset/Supplementary_Material_for_Shan_Virus_A_New_Mimivirus_Isolated_from_the_Stool_of_a_Tunisian_Patient_with_Pneumonia/5125504 %R 10.6084/m9.figshare.5125504.v1 %2 https://ndownloader.figshare.com/files/8712532 %K Human stool %K Shan virus %K Pneumonia %X Objective: Following the isolation of a Marseillevirus from the stool of a healthy young Senegalese and a Mimivirus from a Tunisian patient with pneumonia, we attempted to isolate other giant viruses of amoebae from a large human stool collection. Methods: During the period 2010-2011, a total of 1,605 stool samples, including 115 from Tunisian patients with pneumonia, were cultured on amoebae. We used a recently developed high-throughput isolation system to detect amoebae plaque lysis on agar plates; this method allows for the testing of 100 samples per plate per week. The giant virus was identified by sequencing of genes conserved in Megavirales. Results: A single giant virus, called Shan, was isolated from the stool of a Tunisian patient with pneumonia who responded poorly to antibiotics. This virus has an icosahedral shape typical of members of the family Mimiviridae and a size of 640 ± 10 nm. Phylogenetic analyses showed that Shan virus was classified as a member of Mimivirus lineage C that infects amoebae. Conclusion: Only one isolate was obtained in this study, suggesting that giant viruses of amoebae are rare in human stool. The isolation of Shan virus from a patient with pneumonia brings into question the etiological role of this virus and its subsequent release in stool. %I Karger Publishers