Supplementary Material for: Glycolytic and Mitochondrial Metabolism in Pancreatic Islets from MSG-Treated Obese Rats Subjected to Swimming Training de Carvalho Leite N. Ferreira T.R. Rickli S. Borck P.C. de Freitas Mathias P.C. de Oliveira Emilio H.R. Grassiolli S. 10.6084/m9.figshare.5124163.v1 https://karger.figshare.com/articles/dataset/Supplementary_Material_for_Glycolytic_and_Mitochondrial_Metabolism_in_Pancreatic_Islets_from_MSG-Treated_Obese_Rats_Subjected_to_Swimming_Training/5124163 <b><i>Backgrounds/Aims: </i></b>Obese rats obtained by neonatal monosodium glutamate (MSG) administration present insulin hypersecretion. The metabolic mechanism by which glucose catabolism is coupled to insulin secretion in the pancreatic β-cells from MSG-treated rats is understood. The purpose of this study was to evaluate glucose metabolism in pancreatic islets from MSG-treated rats subjected to swimming training. <b><i>Methods: </i></b>MSG-treated and control (CON) rats swam for 30 minutes (3 times/week) over a period of 10 weeks. Pancreatic islets were isolated and incubated with glucose in the presence of glycolytic or mitochondrial inhibitors. <b><i>Results: </i></b>Swimming training attenuated fat pad accumulation, avoiding changes in the plasma levels of lipids, glucose and insulin in MSG-treated rats. Adipocyte and islet hypertrophy observed in MSG-treated rats were attenuated by exercise. Pancreatic islets from MSG-treated obese rats also showed insulin hypersecretion, greater glucose transporter 2 (GLUT2) expression, increased glycolytic flux and reduced mitochondrial complex III activity. <b><i>Conclusion: </i></b>Swimming training attenuated islet hypertrophy and normalised GLUT2 expression, contributing to a reduction in the glucose responsiveness of pancreatic islets from MSG-treated rats without altering glycolytic flux. However, physical training increased the activity of mitochondrial complex III in pancreatic islets from MSG-treated rats without a subsequent increase in glucose-induced insulin secretion. 2013-02-22 00:00:00 Obesity Exercise Islets Glucose metabolism