%0 Generic %A X., Chen %A Q., Wang %A R., El-Mezayen %A Y., Zhuang %A S.C., Dreskin %D 2012 %T Supplementary Material for: Ara h 2 and Ara h 6 Have Similar Allergenic Activity and Are Substantially Redundant %U https://karger.figshare.com/articles/dataset/Supplementary_Material_for_Ara_h_2_and_Ara_h_6_Have_Similar_Allergenic_Activity_and_Are_Substantially_Redundant/5123869 %R 10.6084/m9.figshare.5123869.v1 %2 https://ndownloader.figshare.com/files/8710015 %K Food allergy %K Peanut %K Ara h 2 %K Ara h 6 %K Allergenic activity %K RBL SX-38 %X Background: The moderately homologous (approx. 60%) proteins Ara h 2 and Ara h 6 are the most potent peanut allergens. This study was designed to define the relative individual contributions of Ara h 2 and Ara h 6 to the overall allergenic activity of a crude peanut extract (CPE). Methods: Ara h 2 and Ara h 6 were removed from CPE by gel filtration chromatography. Ara h 2.01, Ara h 2.02 and Ara h 6 were further purified (>99%). The potency of each allergen and the ability of these allergens to reconstitute the allergenic activity of CPE depleted of Ara h 2 and Ara h 6 was measured with RBL SX-38 cells sensitized with IgE from sensitized peanut allergic patients. Results: The potency of the native proteins were significantly different (p < 0.0001) although not dramatically so, with a rank order of Ara h 2.01 > Ara h 2.02 > Ara h 6. The addition of either purified Ara h 2 or Ara h 6 independently at their original concentration to CPE depleted of both Ara h 2 and Ara h 6 restored 80–100% of the original CPE allergenic activity. Addition of both Ara h 2 and Ara h 6 consistently completely restored the allergenic activity of CPE. Conclusions: These studies indicate that either Ara h 2 or Ara h 6 independently can account for most of the allergenic activity in a CPE and demonstrate important redundancy in the allergenic activity of these related molecules. %I Karger Publishers