%0 Generic %A R.E., Masonbrink %A J.A., Birchler %D 2012 %T Supplementary Material for: Accumulation of Multiple Copies of Maize Minichromosomes %U https://karger.figshare.com/articles/dataset/Supplementary_Material_for_Accumulation_of_Multiple_Copies_of_Maize_Minichromosomes/5123701 %R 10.6084/m9.figshare.5123701.v1 %2 https://ndownloader.figshare.com/files/8709772 %K B chromosome %K Engineered minichromosome %K Maize %X Multiple copies of B chromosomes in maize (Zea mays) can accumulate in the genome using the B chromosome’s accumulation mechanism, specifically nondisjunction at the second pollen mitosis and preferential fertilization of the egg. Using this mechanism, we accumulated 4 different-sized minichromosomes derived from the B chromosome to test the chromosome limits of the cell. The accumulation of normal B chromosomes is associated with multiple phenotypes including white stripes and asymmetric leaf blades, but when minichromosomes are accumulated these symptoms are absent. We also found that multiple B chromosome-derived minichromosomes can coexist with A chromosome-derived minichromosomes. During the years that these experiments were conducted, we found many B chromosome rearrangements and fragments, 2 recoverable A chromosome fragments, and observed a minichromosome breakage-fusion-bridge cycle in roots. %I Karger Publishers