TY - DATA T1 - Supplementary Material for: Enhancement of the Antitumor Effect on Combination Therapy of an Anticancer Drug and Its Antibody against Carcinoembryonic Antigen PY - 2012/04/11 AU - Shibaguchi H. AU - Tsuru H. AU - Kuroki M. UR - https://karger.figshare.com/articles/dataset/Supplementary_Material_for_Enhancement_of_the_Antitumor_Effect_on_Combination_Therapy_of_an_Anticancer_Drug_and_Its_Antibody_against_Carcinoembryonic_Antigen/5123356 DO - 10.6084/m9.figshare.5123356.v1 L4 - https://ndownloader.figshare.com/files/8709082 L4 - https://ndownloader.figshare.com/files/8709085 L4 - https://ndownloader.figshare.com/files/8709088 KW - Human anti-carcinoembryonic antigen antibodies KW - Immunotherapy KW - Anoikis KW - Complement-dependent cytotoxicity KW - Chemotherapy KW - Antibody-dependent cell-mediated cytotoxicity N2 - Background: Carcinoembryonic antigen (CEA) is frequently overexpressed in various types of human cancers and is associated with cell adhesion. There are three possible mechanisms of cancer therapy that employ anti-CEA antibody (Ab): Ab-dependent cell-mediated cytotoxicity (ADCC), complement-dependent cytotoxicity (CDC) or the prevention of CEA interaction with the extracellular matrix and/or intercellular adhesion molecules resulting in anoikis. In this study, the effect of C2-74, a human anti-CEA monoclonal Ab was evaluated. Methods: ADCC, CDC and anoikis assays in combination with C2-74 and an anticancer drug (5-fluorouracil or cisplatin) were investigated using tumor cell lines (MKN-45, MKN-74 and KATO III). In the anoikis assay, other human anti-CEA Abs and mouse anti-CEA-related cell adhesion molecule 6 Abs were also investigated using HLC-1 cells. Results: Additive cytotoxicity was observed when the anticancer drug and C2-74 on tumor cells were combined in the CDC assays, whereas in the anoikis assay, no such additive effect was observed. Anti-CEA-related cell adhesion molecule 6 Abs, but not anti-CEA Abs, accelerated anoikis in HLC-1 cells. Conclusion: A mechanism for the additive antitumor effect when an anticancer drug and C2-74 are combined is indicated mainly by CDC activity but is irrelevant to anoikis in tumor cells. ER -