TY - DATA T1 - Supplementary Material for: Mutations in the Essential Escherichia coli Gene, yqgF, and Their Effects on Transcription PY - 2012/02/21 AU - Iwamoto A. AU - Osawa A. AU - Kawai M. AU - Honda H. AU - Yoshida S. AU - Furuya N. AU - Kato J. UR - https://karger.figshare.com/articles/dataset/Supplementary_Material_for_Mutations_in_the_Essential_i_Escherichia_coli_i_Gene_i_yqgF_i_and_Their_Effects_on_Transcription/5123308 DO - 10.6084/m9.figshare.5123308.v1 L4 - https://ndownloader.figshare.com/files/8709010 L4 - https://ndownloader.figshare.com/files/8709013 KW - Escherichia coli KW - Essential genes KW - NusA KW - Rho KW - Transcription termination N2 - The Escherichia coli yqgF gene is highly conserved across a broad spectrum of bacterial genomes. The gene was first identified as being essential for cell growth during screening for targets for broad-spectrum antibiotics. YqgF is structurally similar to RuvC, a Holliday junction resolvase, but its function has not been established. This study describes the isolation of a temperature-sensitive yqgF mutant, the growth of which was inhibited by rho or nusA multicopy plasmids, indicating that YqgF is involved in transcription. Rho is a global transcription termination factor that acts at Rho-dependent terminator sites, which exist not only at the ends of genes but also within genes. The transcription of genes possessing intragenic, or upstream, Rho-dependent terminators was reduced in temperature-sensitive yqgF mutants. This transcription inhibition was sensitive to the Rho inhibitor, bicyclomycin. In addition, the transcription of mutant tnaA genes defective for upstream Rho-dependent termination was not significantly affected by the yqgF mutation. Taken together, these results suggest that YqgF is involved in anti-termination at Rho-dependent terminators in vivo. ER -