%0 Generic %A M.E., Caceres %A F., Pupilli %A M., Ceccarelli %A P., Vaccino %A V., Sarri %A De Pace C. %A P.G., Cionini %D 2011 %T Supplementary Material for: Cryptic Introgression of Dasypyrum villosum Parental DNA in Wheat Lines Derived from Intergeneric Hybridization %U https://karger.figshare.com/articles/dataset/Supplementary_Material_for_Cryptic_Introgression_of_i_Dasypyrum_villosum_i_Parental_DNA_in_Wheat_Lines_Derived_from_Intergeneric_Hybridization/5123035 %R 10.6084/m9.figshare.5123035.v1 %2 https://ndownloader.figshare.com/files/8708515 %K Cryptic introgression %K Intergeneric hybridization %K Inbred breeding lines %K Triticum aestivum cv. ‘Chinese Spring’ %K Dasypyrum villosum %X Cytogenetic and DNA molecular analyses have been carried out in 3 wheat introgression lines (ILs; CS×V58, CS×V59, and CS×V60) derived from Triticum aestivum cv. ‘Chinese Spring’ (CS) × Dasypyrum villosum(Dv) intergeneric hybridization. All lines, which showed several phenotypic differences compared to CS, had the same chromosome number (2n = 42) and structure as CS, and neither chromosomes nor chromatin from Dv were apparently added to their complement. However, Feulgen/DNA cytophotometry showed that there was more nuclear DNA in the lines than in the parental wheat (by 1.85%, 2.76%, and 1.26% in CS×V58, CS×V59, and CS×V60, respectively). Molecular investigation indicated the presence of Dv DNA in the ILs. AFLP analysis of genomic DNA from the ILs, CS, and Dv detected a total of 120 polymorphic bands, of which 7 (5.8%) were present in some or all the ILs and Dv but were absent in CS. PCR amplification, sequence analysis of amplicons, and Southern blot hybridization confirmed the presence of Dv-specific sequences in each of the ILs. These results indicate cryptic introgression of Dv DNA sequences into the genome of the ILs. Some implications of this finding are discussed. %I Karger Publishers