10.6084/m9.figshare.5123035.v1 Caceres M.E. Caceres M.E. Pupilli F. Pupilli F. Ceccarelli M. Ceccarelli M. Vaccino P. Vaccino P. Sarri V. Sarri V. De Pace C. De Pace C. Cionini P.G. Cionini P.G. Supplementary Material for: Cryptic Introgression of <i>Dasypyrum villosum</i> Parental DNA in Wheat Lines Derived from Intergeneric Hybridization Karger Publishers 2011 Cryptic introgression Intergeneric hybridization Inbred breeding lines Triticum aestivum cv. ‘Chinese Spring’ Dasypyrum villosum 2011-11-18 00:00:00 Dataset https://karger.figshare.com/articles/dataset/Supplementary_Material_for_Cryptic_Introgression_of_i_Dasypyrum_villosum_i_Parental_DNA_in_Wheat_Lines_Derived_from_Intergeneric_Hybridization/5123035 Cytogenetic and DNA molecular analyses have been carried out in 3 wheat introgression lines (ILs; CS×V58, CS×V59, and CS×V60) derived from <i>Triticum aestivum</i> cv. ‘Chinese Spring’ (CS) × <i>Dasypyrum villosum</i><i>(Dv)</i> intergeneric hybridization. All lines, which showed several phenotypic differences compared to CS, had the same chromosome number (2n = 42) and structure as CS, and neither chromosomes nor chromatin from <i>Dv</i> were apparently added to their complement. However, Feulgen/DNA cytophotometry showed that there was more nuclear DNA in the lines than in the parental wheat (by 1.85%, 2.76%, and 1.26% in CS×V58, CS×V59, and CS×V60, respectively). Molecular investigation indicated the presence of <i>Dv</i> DNA in the ILs. AFLP analysis of genomic DNA from the ILs, CS, and <i>Dv</i> detected a total of 120 polymorphic bands, of which 7 (5.8%) were present in some or all the ILs and <i>Dv</i> but were absent in CS. PCR amplification, sequence analysis of amplicons, and Southern blot hybridization confirmed the presence of <i>Dv</i>-specific sequences in each of the ILs. These results indicate cryptic introgression of <i>Dv</i> DNA sequences into the genome of the ILs. Some implications of this finding are discussed.