%0 Generic %A van Durme Y.M.T.A. %A L., Lahousse %A K.M.C., Verhamme %A L., Stolk %A M., Eijgelsheim %A D.W., Loth %A A.G., Uitterlinden %A M.M.B., Breteler %A G.F., Joos %A A., Hofman %D 2011 %T Supplementary Material for: Mendelian Randomization Study of Interleukin-6 in Chronic Obstructive Pulmonary Disease %U https://karger.figshare.com/articles/dataset/Supplementary_Material_for_Mendelian_Randomization_Study_of_Interleukin-6_in_Chronic_Obstructive_Pulmonary_Disease/5122897 %R 10.6084/m9.figshare.5122897.v1 %2 https://ndownloader.figshare.com/files/8708179 %K Chronic obstructive pulmonary disease, epidemiology, inflammation %K Genetics %K Interleukin-6 %X Background: Cross-sectional studies have demonstrated that increased levels of interleukin-6 (IL6) are present in the airways and blood samples of patients with chronic obstructive pulmonary disease (COPD). Objectives: To investigate the association between IL6 and the risk of COPD using a Mendelian randomization approach. Methods: Eight common single-nucleotide polymorphisms (SNPs) in the region of the IL6 gene were genotyped using both TaqMan and Illumina in the Rotterdam Study, a prospective population-based cohort study consisting of 7,983 participants aged 55 years or older, including 928 COPD patients. At baseline, blood was drawn in a random sample of 714 subjects to measure the IL6 plasma level. Analysis of variance, logistic regression, and Cox proportional hazard models – adjusted for age, gender, pack years, and BMI – were used for analyses. Results: High levels of IL6 (>2.4 pg/ml, the highest tertile) were associated with a three-fold increased risk of developing COPD, in comparison to low levels (<1.4 pg/ml, the lowest tertile). The rs2056576 SNP was associated with a 10% increase in the risk of COPD per additional T allele. However, the association was no longer significant after adjustment. No association was found with other common SNPs in the IL6 gene and COPD. Conclusions: Although increased IL6 plasma levels at baseline are associated with the risk of developing COPD during follow-up, there was no strong evidence for an association between common variation in the IL6 gene and the risk of COPD. %I Karger Publishers