Di Meo G.P. A., Perucatti V., Peretti D., Incarnato F., Ciotola L., Liotta T., Raudsepp Di Berardino D. B., Chowdhary L., Iannuzzi Supplementary Material for: The 450-Band Resolution G- and R-Banded Standard Karyotype of the Donkey (<i>Equus asinus</i>, 2n = 62) Donkey chromosomes were earlier characterized separately by C-, G- and R-banding techniques. However, direct comparisons between G- and R-banding patterns have still not been carried out in this species. The present study reports this comparison at the 450-band level by using replication G- and R-banding patterns. Two sets of synchronized lymphocyte cultures were set up to obtain early (GBA+CBA-banding) and late (RBA-banding) BrdU incorporation. Slides were stained with acridine orange and observed under a fluorescence microscope. Reverse GBA+CBA- and RBA-banded karyotypes at the 450-band level were constructed. To verify G- and R-banding patterns in some acrocentric chromosomes, sequential GBA+CBA/Ag-NORs and RBA/Ag-NORs were also performed. The results of CBA-banding patterns obtained in 12 animals from 2 breeds showed a pronounced polymorphism of heterochromatin, especially in EAS1q-prox. Ideogrammatic representations of G- and R-banded karyotypes were constructed using only one common G- and R-banding nomenclature. In the present study both G- and R-banding patterns and relative ideograms are presented as standard karyotype for this species at the 450-band level. C-bands;Donkey;G-bands;R-bands;Standard karyotype 2009-10-27
    https://karger.figshare.com/articles/dataset/Supplementary_Material_for_The_450-Band_Resolution_G-_and_R-Banded_Standard_Karyotype_of_the_Donkey_i_Equus_asinus_i_2n_62_/5120809
10.6084/m9.figshare.5120809.v1