10.1371/journal.ppat.1006454.g006 Jasmin Zischke Jasmin Zischke Panagiota Mamareli Panagiota Mamareli Claudia Pokoyski Claudia Pokoyski Ildar Gabaev Ildar Gabaev Sabine Buyny Sabine Buyny Roland Jacobs Roland Jacobs Christine S. Falk Christine S. Falk Matthias Lochner Matthias Lochner Tim Sparwasser Tim Sparwasser Thomas F. Schulz Thomas F. Schulz Penelope C. Kay-Fedorov Penelope C. Kay-Fedorov Treatment with pUL11Fc affects phosphorylation of the regulatory tyrosines of Lck. Public Library of Science 2017 pUL 11 binding CD 45 substrate Lck TCR CD 45 pUL 11 CD 45 phosphatase PBMC IL -10 production HCMV infection cytomegalovirus glycoprotein pUL 11 acts pUL 11. Supernatants pUL 11-induced changes T cell IL -10 secretion signal strength influence T cell development T cell IL -10 secretion Human Cytomegalovirus T cell proliferation T cells 2017-06-19 17:45:02 Figure https://plos.figshare.com/articles/figure/Treatment_with_pUL11Fc_affects_phosphorylation_of_the_regulatory_tyrosines_of_Lck_/5119636 <p>PBMC were incubated with anti-CD3 (OKT3) in the presence or absence of UL11Fc and Fc at the indicated concentrations for three days. Cells were then labelled extracellularly with anti-CD3 and anti-CD4 and intracellularly with anti-Lck pY505 or anti-Lck pY394. The experiment was repeated three times using cells from different donors. Mean fluorescence intensities are shown for Y505 (i) and Y394 (ii) phosphorylation of live CD3+CD4+ cells, fold increases in geometric mean fluorescent intensity normalised to anti-CD3 treated cells are indicated. A) depicts one representative experiment (values for UL11 treated cells). B) shows fold increases in phosphorylation over all three experiments for UL11Fc and Fc treated cells. Error bars indicate standard deviation. Statistical significance of differences between groups was determined by ANOVA. i)*p = 0.0014 for the difference in Y505 phosphorylation between pUL11Fc and Fc treated cells. ii)*p = 0.0043 for the difference in Y394 phosphorylation between pUL11Fc and Fc treated cells.</p>