10.1371/journal.ppat.1006454.g006
Jasmin Zischke
Jasmin
Zischke
Panagiota Mamareli
Panagiota
Mamareli
Claudia Pokoyski
Claudia
Pokoyski
Ildar Gabaev
Ildar
Gabaev
Sabine Buyny
Sabine
Buyny
Roland Jacobs
Roland
Jacobs
Christine S. Falk
Christine
S. Falk
Matthias Lochner
Matthias
Lochner
Tim Sparwasser
Tim
Sparwasser
Thomas F. Schulz
Thomas
F. Schulz
Penelope C. Kay-Fedorov
Penelope
C. Kay-Fedorov
Treatment with pUL11Fc affects phosphorylation of the regulatory tyrosines of Lck.
Public Library of Science
2017
pUL 11 binding
CD 45 substrate Lck
TCR
CD 45
pUL 11
CD 45 phosphatase
PBMC
IL -10 production
HCMV infection
cytomegalovirus glycoprotein pUL 11 acts
pUL 11. Supernatants
pUL 11-induced changes
T cell IL -10 secretion
signal strength influence T cell development
T cell IL -10 secretion Human Cytomegalovirus
T cell proliferation
T cells
2017-06-19 17:45:02
Figure
https://plos.figshare.com/articles/figure/Treatment_with_pUL11Fc_affects_phosphorylation_of_the_regulatory_tyrosines_of_Lck_/5119636
<p>PBMC were incubated with anti-CD3 (OKT3) in the presence or absence of UL11Fc and Fc at the indicated concentrations for three days. Cells were then labelled extracellularly with anti-CD3 and anti-CD4 and intracellularly with anti-Lck pY505 or anti-Lck pY394. The experiment was repeated three times using cells from different donors. Mean fluorescence intensities are shown for Y505 (i) and Y394 (ii) phosphorylation of live CD3+CD4+ cells, fold increases in geometric mean fluorescent intensity normalised to anti-CD3 treated cells are indicated. A) depicts one representative experiment (values for UL11 treated cells). B) shows fold increases in phosphorylation over all three experiments for UL11Fc and Fc treated cells. Error bars indicate standard deviation. Statistical significance of differences between groups was determined by ANOVA. i)*p = 0.0014 for the difference in Y505 phosphorylation between pUL11Fc and Fc treated cells. ii)*p = 0.0043 for the difference in Y394 phosphorylation between pUL11Fc and Fc treated cells.</p>