HCMV infected epithelial cells induce IL-10 secretion from PBMC.
Jasmin Zischke
Panagiota Mamareli
Claudia Pokoyski
Ildar Gabaev
Sabine Buyny
Roland Jacobs
Christine S. Falk
Matthias Lochner
Tim Sparwasser
Thomas F. Schulz
Penelope C. Kay-Fedorov
10.1371/journal.ppat.1006454.g002
https://plos.figshare.com/articles/figure/HCMV_infected_epithelial_cells_induce_IL-10_secretion_from_PBMC_/5119606
<p>PBMC were incubated for 48h alone or with 50nM UL11Fc or Fc as a control, or with RPE cells that were uninfected (RPE) or infected with wild type HCMV (WT RPE) or ΔUL11 HCMV (ΔUL11 RPE) at the indicated ratios and in the absence or presence of anti-CD3 (OKT3). The presence of secreted IL-10 was measured by ELISA after 2 days. The experiment was performed three times, using PBMC from different donors. (A) One representative experiment is shown, with IL-10 concentration in pg/ml. Error bars show standard deviation of technical replicates. (B) Mean fold increases in IL-10 concentration over the value for uninfected RPE cells incubated with PBMC at a ratio of 1:5 in the presence of anti-CD3 are shown for the three experiments combined. Error bars show standard deviation. Statistical significance of differences between groups was determined by ANOVA. *p = 0.0005 for the difference in IL-10 expression between supernatants from cultures containing wild type HCMV and those containing ΔUL11 HCMV infected cells.</p>
2017-06-19 17:45:02
pUL 11 binding
CD 45 substrate Lck
TCR
CD 45
pUL 11
CD 45 phosphatase
PBMC
IL -10 production
HCMV infection
cytomegalovirus glycoprotein pUL 11 acts
pUL 11. Supernatants
pUL 11-induced changes
T cell IL -10 secretion
signal strength influence T cell development
T cell IL -10 secretion Human Cytomegalovirus
T cell proliferation
T cells