10.1371/journal.pone.0179327.g002 Rahul Sampath Rahul Sampath Nathan W. Cummins Nathan W. Cummins Sekar Natesampillai Sekar Natesampillai Gary D. Bren Gary D. Bren Thomas D. Chung Thomas D. Chung Jason Baker Jason Baker Keith Henry Keith Henry Amélie Pagliuzza Amélie Pagliuzza Andrew D. Badley Andrew D. Badley Procaspase 8 inducing drugs in acute <i>in vitro</i> HIV infection model. Public Library of Science 2017 vivo patient cells HIV CD 4 T cells cytotoxic protein Casp 8p procaspase 8 levels cell-associated HIV DNA levels HIV reactivation increase Casp 8p 9 drugs Primary CD 4 T cells cell-associated HIV DNA HIV protease cleaving procaspase 8. procaspase 8 expression Casp 8p levels HIV IIIb infection model HIV DNA levels Screening 1277 FDA cell death reactivating cells 2017-06-19 17:37:15 Figure https://plos.figshare.com/articles/figure/Procaspase_8_inducing_drugs_in_acute_i_in_vitro_i_HIV_infection_model_/5119003 <p><b>A)</b> Primary uninfected CD4 cells from 3 donors were infected with HIV IIIB and then incubated with procaspase 8 inducing drugs for 48hours with RAL and TDF. ATP, HIV DNA by qPCR <b>(B)</b>, and supernatant P24 <b>(D)</b> were assessed. <b>C)</b> The degree of procaspase 8 expression induction by ELISA was compared between drugs which reduced HIV DNA or did not reduce HIV DNA in panel (B). Depicted are means (SD). E) Pearson correlation coefficient between the change in cell associated HIV DNA and change in supernatant p24 was assessed.</p>