%0 Journal Article
%A Arora, Neha
%A Patel, Alok
%A Sharma, Meenakshi
%A Mehtani, Juhi
%A Pruthi, Parul A.
%A Pruthi, Vikas
%A Mohan Poluri, Krishna
%D 2017
%T Insights into the Enhanced Lipid Production Characteristics of a
Fresh Water Microalga under High Salinity Conditions
%U https://acs.figshare.com/articles/journal_contribution/Insights_into_the_Enhanced_Lipid_Production_Characteristics_of_a_Fresh_Water_Microalga_under_High_Salinity_Conditions/5110771
%R 10.1021/acs.iecr.7b00841.s001
%2 https://ndownloader.figshare.com/files/8675791
%K lipid content
%K IITRIND
%K seawater
%K FAME
%K halotolerant algal strains
%K acid
%K EN
%K ASTM
%K reactive oxygen species
%K Fresh Water Microalga
%K microalga
%K algal biodiesel production
%K BBM
%K High Salinity Conditions Bioprospecting
%K H 2 O 2
%K saline
%K Enhanced Lipid Production Characteristics
%K stress
%K analysis
%K salinity
%X Bioprospecting of
microalgae capable of growing and accumulating high amounts of lipids
in high salinity conditions such as seawater can substantially improve
the economic vaibaility of algal biodiesel production. In view of
this, a fresh water microalga, Scenedesmus sp. IITRIND2,
was cultivated under saline conditions to assess its halotolerant
behavior and potential as biodiesel feedstock. The microalga efficiently
adapted to 100% seawater salinity, enhanced its lipid content by 52%,
thus yielded ∼3.2 fold higher lipid productivity as compared
to the Bold’s basal media (BBM). The increase in the lipid
content was balanced by a sharp decrease in its protein and carbohydrate
content. Biochemical analysis evidenced that salinity induced oxidative
stress resulted in reduced levels of photosynthetic pigments, elevated
levels of reactive oxygen species (H2O2, thiobarbituric
acid reactive substances), osmolytes (proline, glycine betaine), and
activity of antioxidant enzymes (catalase, ascorbate peroxidase).
These studies suggested that microalga efficiently modulated its metabolic
flexibility in order to acclatamize the salanity induced stress. Further,
the FAME analysis revealed the dominance of C14:0, C16:0, C18:0, C18:1,
and C18:2 fatty acids under halotolerant conditions, and the properties
of the resulting biodiesel were in compliance with ASTM (American
Society for Testing Materials) D6751 and EN 14214 (European) fuel
standards. These results consolidate that the lipid augmented halotolerant
algal strains capable of growing in saline/seawater can be formulated
as environmental sustainable and economic viable sources for biodiesel
production.
%I ACS Publications