Supplementary Material for: Systems biology of the 2-cell mouse embryo Latham K.E. Radford E.E. Evsikov A.V. de Vries W.N. Peaston A.E. Fancher K.S. Chen F.H. Blake J.A. Bult C.J. Solter D. Knowles B.B. 10.6084/m9.figshare.5104417.v1 https://karger.figshare.com/articles/dataset/Supplementary_Material_for_Systems_biology_of_the_2-cell_mouse_embryo/5104417 The transcriptome of the 2-cell mouse embryo was analyzed to provide insight into the molecular networks at play during nuclear reprogramming and embryonic genome activation. Analysis of ESTs from a 2-cell cDNA library identified nearly 4,000 genes, over half of which have not been previously studied. Transcripts of mobile elements, especially those of LTR retrotransposons, are abundantly represented in 2-cell embryos, suggesting their possible role in introducing genomic variation, and epigenetic restructuring of the embryonic genome. Analysis of Gene Ontology of the 2-cell-stage expressed genes outlines the major biological processes that guide the oocyte-to-embryo transition. These results provide a foundation for understanding molecular control at the onset of mammalian development. 2004-07-14 00:00:00 Medicine