Supplementary Material for: Systems biology of the 2-cell mouse embryo
Latham K.E.
Radford E.E.
Evsikov A.V.
de Vries W.N.
Peaston A.E.
Fancher K.S.
Chen F.H.
Blake J.A.
Bult C.J.
Solter D.
Knowles B.B.
10.6084/m9.figshare.5104417.v1
https://karger.figshare.com/articles/dataset/Supplementary_Material_for_Systems_biology_of_the_2-cell_mouse_embryo/5104417
The transcriptome of the 2-cell mouse embryo was analyzed to provide insight into the molecular networks at play during nuclear reprogramming and embryonic genome activation. Analysis of ESTs from a 2-cell cDNA library identified nearly 4,000 genes, over half of which have not been previously studied. Transcripts of mobile elements, especially those of LTR retrotransposons, are abundantly represented in 2-cell embryos, suggesting their possible role in introducing genomic variation, and epigenetic restructuring of the embryonic genome. Analysis of Gene Ontology of the 2-cell-stage expressed genes outlines the major biological processes that guide the oocyte-to-embryo transition. These results provide a foundation for understanding molecular control at the onset of mammalian development.
2004-07-14 00:00:00
Medicine