10.1371/journal.pone.0177146.g003 Lars Henning Schmidt Lars Henning Schmidt Caroline Brand Caroline Brand Janine Stucke-Ring Janine Stucke-Ring Christoph Schliemann Christoph Schliemann Torsten Kessler Torsten Kessler Saliha Harrach Saliha Harrach Michael Mohr Michael Mohr Dennis Görlich Dennis Görlich Alessandro Marra Alessandro Marra Ludger Hillejan Ludger Hillejan Carsten Müller-Tidow Carsten Müller-Tidow Georg Lenz Georg Lenz Eva Wardelmann Eva Wardelmann Rainer Wiewrodt Rainer Wiewrodt Wolfgang E. Berdel Wolfgang E. Berdel Christian Schwöppe Christian Schwöppe Wolfgang Hartmann Wolfgang Hartmann <i>In vivo</i> therapeutic activity of systemic tTF-NGR against CD13+ A549 tumor xenografts. Public Library of Science 2017 Results CD 13 expression 270 NSCLC patients lung cancer histologies CD 13-targeted tissue factor tTF-NGR NSCLC patients CD 13 expression Microarray-based mRNA analysis CD 13 protein expression 549 lung carcinoma xenotransplants CD 13-targeted fusion protein tTF-NGR non-small cell lung cancer Background Aminopeptidase N CD 13 mRNA expression NSCLC study cohort KM plotter database CD 13 III 549 tumor growth 2017-06-12 17:31:30 Figure https://plos.figshare.com/articles/figure/_i_In_vivo_i_therapeutic_activity_of_systemic_tTF-NGR_against_CD13_A549_tumor_xenografts_/5101654 <p>To investigate CD13 expression flow cytometry was performed with a monoclonal PE-labeled anti-CD13 antibody. CD13 expression was found in 47% of the A549 lung cancer cells (green, control; purple, CD13) (<b>Fig. 3A</b>). Following treatment with tTF-NGR (1 mg tTF-NGR/kg x4 (arrows); i.v.; n = 4 CD-1 nude mice) tumor growth of subcutaneous A549 xenotransplants was reduced as compared to the saline control group (n = 6) CD-1 nude mice (<b>Fig. 3B)</b>. The CD13 expression in subcutaneous A549 xenotransplant is demonstrated by immunofluorescence; since the antibodies used for CD13 and CD31 staining are species-specific for human CD13 and CD31, vascular and perivascular staining were not assayed in the xenografts (<b>Fig. 3C</b>).</p>