10.1371/journal.pone.0177146.g003
Lars Henning Schmidt
Lars
Henning Schmidt
Caroline Brand
Caroline
Brand
Janine Stucke-Ring
Janine
Stucke-Ring
Christoph Schliemann
Christoph
Schliemann
Torsten Kessler
Torsten
Kessler
Saliha Harrach
Saliha
Harrach
Michael Mohr
Michael
Mohr
Dennis Görlich
Dennis
Görlich
Alessandro Marra
Alessandro
Marra
Ludger Hillejan
Ludger
Hillejan
Carsten Müller-Tidow
Carsten
Müller-Tidow
Georg Lenz
Georg
Lenz
Eva Wardelmann
Eva
Wardelmann
Rainer Wiewrodt
Rainer
Wiewrodt
Wolfgang E. Berdel
Wolfgang E.
Berdel
Christian Schwöppe
Christian
Schwöppe
Wolfgang Hartmann
Wolfgang
Hartmann
<i>In vivo</i> therapeutic activity of systemic tTF-NGR against CD13+ A549 tumor xenografts.
Public Library of Science
2017
Results CD 13 expression
270 NSCLC patients
lung cancer histologies
CD 13-targeted tissue factor tTF-NGR
NSCLC patients
CD 13 expression
Microarray-based mRNA analysis
CD 13 protein expression
549 lung carcinoma xenotransplants
CD 13-targeted fusion protein tTF-NGR
non-small cell lung cancer Background Aminopeptidase N
CD 13 mRNA expression
NSCLC study cohort
KM plotter database
CD 13
III
549 tumor growth
2017-06-12 17:31:30
Figure
https://plos.figshare.com/articles/figure/_i_In_vivo_i_therapeutic_activity_of_systemic_tTF-NGR_against_CD13_A549_tumor_xenografts_/5101654
<p>To investigate CD13 expression flow cytometry was performed with a monoclonal PE-labeled anti-CD13 antibody. CD13 expression was found in 47% of the A549 lung cancer cells (green, control; purple, CD13) (<b>Fig. 3A</b>). Following treatment with tTF-NGR (1 mg tTF-NGR/kg x4 (arrows); i.v.; n = 4 CD-1 nude mice) tumor growth of subcutaneous A549 xenotransplants was reduced as compared to the saline control group (n = 6) CD-1 nude mice (<b>Fig. 3B)</b>. The CD13 expression in subcutaneous A549 xenotransplant is demonstrated by immunofluorescence; since the antibodies used for CD13 and CD31 staining are species-specific for human CD13 and CD31, vascular and perivascular staining were not assayed in the xenografts (<b>Fig. 3C</b>).</p>