10.6084/m9.figshare.5091928
Valerio Licursi
Valerio
Licursi
Mariangela Cestelli Guidi
Mariangela Cestelli
Guidi
Giorgia Del Vecchio
Giorgia
Del Vecchio
Cecilia Mannironi
Cecilia
Mannironi
Carlo Presutti
Carlo
Presutti
Roberto Amendola
Roberto
Amendola
Rodolfo Negri
Rodolfo
Negri
Leptin induction following irradiation is a conserved feature in mammalian epithelial cells and tissues
Taylor & Francis Group
2017
Leptin
ionizing radiation
radiotherapy
miRNA
transcriptional response to irradiation
2017-07-10 10:26:53
Dataset
https://tandf.figshare.com/articles/dataset/Leptin_induction_following_irradiation_is_a_conserved_feature_in_mammalian_epithelial_cells_and_tissues/5091928
<p><b>Purpose:</b> Leptin (LEP) is a peptide hormone with multiple physiological functions. Besides its systemic actions, it has important peripheral roles such as a mitogen action on keratinocytes following skin lesions. We previously showed that LEP mRNA is significantly induced in response to neutron irradiation in mouse skin and that the protein increases in the irradiated epidermis and in the related subcutaneous adipose tissue. In this work, we investigated the post-transcriptional regulation of LEP by miRNAs and the conservation of LEP’s role in radiation response in human cells.</p> <p><b>Methods:</b> We used microarray analysis and real-time polymerase chain reaction (RT-PCR) to analyze modulation of miRNAs potentially targeting LEP in mouse skin following irradiation and bioinformatic analysis of transcriptome of irradiated human cell lines and cancer tissues from radiotherapy-treated patients to evaluate LEP expression.</p> <p><b>Results and conclusions:</b> We show that a network of miRNAs potentially targeting LEP mRNA is modulated in irradiated mouse skin and that LEP itself is significantly modulated by irradiation in human epithelial cell lines and in breast cancer tissues from radiotherapy-treated patients. These results confirm and extend the previous evidence that LEP has a general and important role in the response of mammalian cells to irradiation.</p>