Azeem, Waqas Hellem, Margrete Reime Roger Olsen, Jan Hua, Yaping Marvyin, Kristo Qu, Yi Lin, Biaoyang Ke, Xisong Margrete Øyan, Anne Kalland, Karl-Henning ARE reporter response in mesenchymal type cells. <p>(A) FACS analysis of mCherry signals in EPT3-PT1-AR-241B cells. Cells were grown in androgen free medium and treated with different AR agonists (1 nM R1881 and 1 μM DHT), steroids (10 nM dexamethasone, 10 nM progesterone and 1 μM β-estradiol), AR antagonist (10 μM enzalutamide) and anti-androgen (10 μM abiraterone). (B) Texas red (Tx-Red) indirect immunofluorescent detection of AR in EPT3-PT1-AR-241B cells. The cells were treated with ± 1 nM R1881 for 24 hours.</p> cell lines transduced;luciferase reporter genes;reporter activity;LNCaP cells;luminal differentiation transcriptome;tumorigenic mesenchymal progeny cells;androgen synthesis inhibitor;lentiviral vector backbones;epithelial cell lines;prostate cells;reporter vectors;AR target gene activation;background reporter activity;mesenchymal type cells;prostate cancer;CRPC;prostate cancer cells;androgen receptor activity;EP 156T cells;androgen response element 2017-06-01
    https://plos.figshare.com/articles/figure/ARE_reporter_response_in_mesenchymal_type_cells_/5059387
10.1371/journal.pone.0177861.g007