Wu, Zhifang Wang, Xiaokan Wang, Zhe Shao, Changyu Jin, Xiaoting Zhang, Leiqing Pan, Haihua Tang, Ruikang Fu, Baiping Self-Etch Adhesive as a Carrier for ACP Nanoprecursors to Deliver Biomimetic Remineralization Lab biomineralization should be carried out in an actual clinical practice. This study evaluated self-etch adhesive as a carrier for amorphous calcium phosphate (ACP) nanoprecursors to continuously deliver biomimetic remineralization of self-assembly type I collagen and demineralized dentin. Si-containing ACP particles (Si-ACP) stabilized with polyaspartic acid (PAsp) were synthesized and characterized by transmission electron microscopy (TEM), scanning electron microscopy–energy-dispersive X-ray spectroscopy, Fourier transform infrared analysis, X-ray powder diffractometry, and X-ray phototelectron spectroscopy. The biomimetic remineralization of single-layer reconstituted type I collagen fibrils and demineralized dentin was analyzed by using two one-bottle self-etch dentin adhesives (Clearfil S3 Bond (S3), Kurraray-Noritake; Adper Easy One (AEO), 3 M ESPE) as a carrier loaded (or not, in the case of the control) with 25 wt % of Si-ACP particles. In vitro cytotoxicity assessed by the Cell Counting Kit-8 indicated that the Si-ACP particles had no adverse effect on cell viability. The capacity for Ca and P ions release from cured Si-ACP-containing adhesives (S3, AEO) was evaluated by inductively coupled plasma-atomic emission spectrometry, revealing the successively increasing release of Ca and P ions for 28 days. The intra- and extrafibrillar remineralization of type I collagen and demineralized dentin was confirmed by TEM and selected-area electron diffraction when the adhesives were used as a carrier loaded with Si-ACP particles. Therefore, we propose self-etch adhesive as a novel carrier for ACP nanoprecursors to continuously deliver biomimetic remineralization. ESPE;Clearfil S 3 Bond;Si-containing ACP particles;plasma-atomic emission spectrometry;X-ray phototelectron spectroscopy;nanoprecursor;AEO;demineralized dentin;carrier;collagen;TEM;one-bottle self-etch dentin adhesives;Si-ACP particles;transmission electron microscopy;Biomimetic Remineralization Lab biomineralization;single-layer reconstituted type;selected-area electron diffraction;biomimetic remineralization;P ions release;X-ray powder diffractometry 2017-05-19
    https://acs.figshare.com/articles/journal_contribution/Self-Etch_Adhesive_as_a_Carrier_for_ACP_Nanoprecursors_to_Deliver_Biomimetic_Remineralization/5024822
10.1021/acsami.7b01719.s001