Example video for raw, straightened, and tracked data. Jeffrey P. Nguyen Ashley N. Linder George S. Plummer Joshua W. Shaevitz Andrew M. Leifer 10.1371/journal.pcbi.1005517.s001 https://plos.figshare.com/articles/media/Automatically_tracking_neurons_in_a_moving_and_deforming_brain/5018993 <p>Left: Raw video feed from high magnification RFP video. The imaging plane is scanning up and down through the volume of the worm’s brain. The recording is shown at 1/2× speed and the time elapsed is indicated in the bottom left. Middle: Maximum intensity projection of each volume is shown after Worm Centerline Tracking and Straightening. Right: Locations of neurons are shown at the end of the pipeline (after Neuron Registration Vector Encoding, Clustering and Error Correction). Each color represents a different tracked neuron. All neurons from the volume are shown overlaid on a raw image of the middle plane of each volume. Note a light flash used for time synchronization is visible around <i>t</i> = 13s.</p> <p>(MP4)</p> 2017-05-18 17:28:04 8 minute recording Neuron Registration Vector Encoding approach non-rigid point-set registration deforming brain Advances Neuron Registration Vector Encoding computer vision pipeline thin-plate spline interpolation neuron activity traces 3 D whole-brain calcium imaging recordings time-independent machine-learning approach