Example video for raw, straightened, and tracked data.
Jeffrey P. Nguyen
Ashley N. Linder
George S. Plummer
Joshua W. Shaevitz
Andrew M. Leifer
10.1371/journal.pcbi.1005517.s001
https://plos.figshare.com/articles/media/Automatically_tracking_neurons_in_a_moving_and_deforming_brain/5018993
<p>Left: Raw video feed from high magnification RFP video. The imaging plane is scanning up and down through the volume of the worm’s brain. The recording is shown at 1/2× speed and the time elapsed is indicated in the bottom left. Middle: Maximum intensity projection of each volume is shown after Worm Centerline Tracking and Straightening. Right: Locations of neurons are shown at the end of the pipeline (after Neuron Registration Vector Encoding, Clustering and Error Correction). Each color represents a different tracked neuron. All neurons from the volume are shown overlaid on a raw image of the middle plane of each volume. Note a light flash used for time synchronization is visible around <i>t</i> = 13s.</p> <p>(MP4)</p>
2017-05-18 17:28:04
8 minute recording
Neuron Registration Vector Encoding approach
non-rigid point-set registration
deforming brain Advances
Neuron Registration Vector Encoding
computer vision pipeline
thin-plate spline interpolation
neuron activity traces
3 D
whole-brain calcium imaging recordings
time-independent machine-learning approach