10.6084/m9.figshare.c.3775388_D1.v1 Manoj Kannan Manoj Kannan Jingfeng Li Jingfeng Li Sarah Fritz Sarah Fritz Kathryn Husarek Kathryn Husarek Jonathan Sanford Jonathan Sanford Teresa Sullivan Teresa Sullivan Pawan Tiwary Pawan Tiwary Wenfeng An Wenfeng An Jef Boeke Jef Boeke David Symer David Symer Additional file 1: of Dynamic silencing of somatic L1 retrotransposon insertions reflects the developmental and cellular contexts of their genomic integration Springer Nature 2017 knockout cells Table T 2. De sequences retrotransposed SAGE tags L 1 retrotransposons DNMT 3b methyltransferase DNMT 1 histone acetylation beta-lactamase reporter assay cell populations PDF 18544 kb Figure S 5. Lack cancer cells Figure S 2. Schematics L 1 template sequence Figure S 6. Variable L 1 reporter expression antisense orientation colorectal cancer HCT HeLa cells Table T 1. Oligonucleotides genomic integration Figure S 1. Schematic L 1 integrant features flow cytometry analysis cytosine methylation L 1 retrotransposon insertions Table T 3. Expression status L 1. Figure S 3. L 1 reporter insertions Figure S 4. Dense maintenance methylation 2017-05-10 05:00:00 Journal contribution https://springernature.figshare.com/articles/journal_contribution/Additional_file_1_of_Dynamic_silencing_of_somatic_L1_retrotransposon_insertions_reflects_the_developmental_and_cellular_contexts_of_their_genomic_integration/4990169 Figure S1. Schematic of beta-lactamase reporter assay. Figure S2. Schematics of newly integrated sequences retrotransposed by L1. Figure S3. Standardized blue and green cell populations as controls for flow cytometry analysis. Figure S4. Dense maintenance methylation of pre-existing L1 retrotransposons in cultured human colorectal cancer (HCT116) cells is reduced dramatically in DNMT1 and DNMT3b methyltransferase double knockout cells. Figure S5. Lack of cytosine methylation at silenced, de novo L1 reporter insertions in cultured HeLa cells. Figure S6. Variable L1 reporter expression in cultured cancer cells is associated with changes in histone acetylation. Table T1. Oligonucleotides used in this study. Table T2. De novo L1 integrant features. Table T3. Expression status of predicted SAGE tags from consensus human L1 template sequence in sense and antisense orientation. (PDF 18544 kb)