10.6084/m9.figshare.c.3775388_D1.v1
Manoj Kannan
Manoj
Kannan
Jingfeng Li
Jingfeng
Li
Sarah Fritz
Sarah
Fritz
Kathryn Husarek
Kathryn
Husarek
Jonathan Sanford
Jonathan
Sanford
Teresa Sullivan
Teresa
Sullivan
Pawan Tiwary
Pawan
Tiwary
Wenfeng An
Wenfeng
An
Jef Boeke
Jef
Boeke
David Symer
David
Symer
Additional file 1: of Dynamic silencing of somatic L1 retrotransposon insertions reflects the developmental and cellular contexts of their genomic integration
Springer Nature
2017
knockout cells
Table T 2. De
sequences retrotransposed
SAGE tags
L 1 retrotransposons
DNMT 3b methyltransferase
DNMT 1
histone acetylation
beta-lactamase reporter assay
cell populations
PDF 18544 kb
Figure S 5. Lack
cancer cells
Figure S 2. Schematics
L 1 template sequence
Figure S 6. Variable L 1 reporter expression
antisense orientation
colorectal cancer
HCT
HeLa cells
Table T 1. Oligonucleotides
genomic integration Figure S 1. Schematic
L 1 integrant features
flow cytometry analysis
cytosine methylation
L 1 retrotransposon insertions
Table T 3. Expression status
L 1. Figure S 3.
L 1 reporter insertions
Figure S 4. Dense maintenance methylation
2017-05-10 05:00:00
Journal contribution
https://springernature.figshare.com/articles/journal_contribution/Additional_file_1_of_Dynamic_silencing_of_somatic_L1_retrotransposon_insertions_reflects_the_developmental_and_cellular_contexts_of_their_genomic_integration/4990169
Figure S1. Schematic of beta-lactamase reporter assay. Figure S2. Schematics of newly integrated sequences retrotransposed by L1. Figure S3. Standardized blue and green cell populations as controls for flow cytometry analysis. Figure S4. Dense maintenance methylation of pre-existing L1 retrotransposons in cultured human colorectal cancer (HCT116) cells is reduced dramatically in DNMT1 and DNMT3b methyltransferase double knockout cells. Figure S5. Lack of cytosine methylation at silenced, de novo L1 reporter insertions in cultured HeLa cells. Figure S6. Variable L1 reporter expression in cultured cancer cells is associated with changes in histone acetylation. Table T1. Oligonucleotides used in this study. Table T2. De novo L1 integrant features. Table T3. Expression status of predicted SAGE tags from consensus human L1 template sequence in sense and antisense orientation. (PDF 18544 kb)