Single-molecule protein identification by sub-nanopore sensors - Fig 2 Mikhail Kolmogorov Eamonn Kennedy Zhuxin Dong Gregory Timp Pavel A. Pevzner 10.1371/journal.pcbi.1005356.g002 https://plos.figshare.com/articles/figure/Single-molecule_protein_identification_by_sub-nanopore_sensors_-_Fig_2/4987229 <p>(a) An example of a pore current trace acquired from a denatured H3.3 histone translocating through sub-nanopore with a nominal diameter of 0.5-nm. (b) The bottom trace is a magnified view of a 600 ms region of a top trace, showing a current blockade associated with the translocation of a single protein molecule. In the figure, higher values correspond to larger blockade currents. Blockades, associated with the translocation of single proteins were identified as regions with fluctuations five standard deviations above the noise level and with duration > 1 ms.); and then the raw current <i>I</i> was converted into <i>fractional blockade current</i>.</p> 2017-05-09 17:24:46 single-molecule protein identification sub-nanopore sensors identification p-values Single-molecule protein identification nanopore technology blockade signal top-down mass spectrometry ion counts fragmentation event DNA sequencing SMPI protein databases