Single-molecule protein identification by sub-nanopore sensors - Fig 2
Mikhail Kolmogorov
Eamonn Kennedy
Zhuxin Dong
Gregory Timp
Pavel A. Pevzner
10.1371/journal.pcbi.1005356.g002
https://plos.figshare.com/articles/figure/Single-molecule_protein_identification_by_sub-nanopore_sensors_-_Fig_2/4987229
<p>(a) An example of a pore current trace acquired from a denatured H3.3 histone translocating through sub-nanopore with a nominal diameter of 0.5-nm. (b) The bottom trace is a magnified view of a 600 ms region of a top trace, showing a current blockade associated with the translocation of a single protein molecule. In the figure, higher values correspond to larger blockade currents. Blockades, associated with the translocation of single proteins were identified as regions with fluctuations five standard deviations above the noise level and with duration > 1 ms.); and then the raw current <i>I</i> was converted into <i>fractional blockade current</i>.</p>
2017-05-09 17:24:46
single-molecule protein identification
sub-nanopore sensors
identification p-values
Single-molecule protein identification
nanopore technology
blockade signal
top-down mass spectrometry
ion counts
fragmentation event
DNA sequencing
SMPI
protein databases