10.6084/m9.figshare.4970054.v1
Haase N.
Haase
N.
Rüder C.
Rüder
C.
Haase H.
Haase
H.
Kamann S.
Kamann
S.
Kouno M.
Kouno
M.
Morano I.
Morano
I.
Dechend R.
Dechend
R.
Zohlnhöfer D.
Zohlnhöfer
D.
Haase T.
Haase
T.
Supplementary Material for: Protective Function of Ahnak1 in Vascular Healing after Wire Injury
Karger Publishers
2017
Vascular remodeling
Neointima formation
Endothelialization
2017-05-04 06:46:27
Dataset
https://karger.figshare.com/articles/dataset/Supplementary_Material_for_Protective_Function_of_Ahnak1_in_Vascular_Healing_after_Wire_Injury/4970054
<p><b><i>Aim:</i></b> Vascular remodeling following injury substantially
accounts for restenosis and adverse clinical outcomes. In this study,
we investigated the role of the giant scaffold protein Ahnak1 in
vascular healing after endothelial denudation of the murine femoral
artery. <b><i>Methods:</i></b> The spatiotemporal expression pattern of
Ahnak1 and Ahnak2 was examined using specific antibodies and real-time
quantitative PCR. Following wire-mediated endothelial injury of
Ahnak1-deficient mice and wild-type (WT) littermates, the processes of
vascular healing were analyzed. <b><i>Results:</i></b> Ahnak1 and Ahnak2
showed a mutually exclusive vascular expression pattern, with Ahnak1
being expressed in the endothelium and Ahnak2 in the medial cells in
naïve WT arteries. After injury, a marked increase of Ahnak1- and
Ahnak2-positive cells at the lesion site became evident. Both proteins
showed a strong upregulation in neointimal cells 14 days after injury.
Ahnak1-deficient mice showed delayed vascular healing and dramatically
impaired re-endothelialization that resulted in prolonged adverse
vascular remodeling, when compared to the WT littermates. <b><i>Conclusion:</i></b>
The large scaffold and adaptor proteins Ahnak1 and Ahnak2 exhibit
differential expression patterns and functions in naïve and injured
arteries. Ahnak1 plays a nonredundant protective role in vascular
healing.</p>