10.1371/journal.ppat.1001133.g004 Gordon Y. C. Cheung Gordon Y. C. Cheung Kevin Rigby Kevin Rigby Rong Wang Rong Wang Shu Y. Queck Shu Y. Queck Kevin R. Braughton Kevin R. Braughton Adeline R. Whitney Adeline R. Whitney Martin Teintze Martin Teintze Frank R. DeLeo Frank R. DeLeo Michael Otto Michael Otto Neutrophil lysis by <i>S. epidermidis</i> PSM peptides and culture filtrates of PSMδ-expression strains. Public Library of Science 2013 lysis psm peptides filtrates 2013-02-20 23:01:30 Figure https://plos.figshare.com/articles/figure/_Neutrophil_lysis_by_S_epidermidis_PSM_peptides_and_culture_filtrates_of_PSM_expression_strains_/496312 <p>(A) Neutrophil (PMN) lysis by synthetic, N-formylated PSM peptides at 10 µg/ml was determined by measuring release of LDH (incubation time, 1 h). PSMα3 (<i>S. aureus</i>) was used as a comparison at the same concentration. (B) Neutrophil lysis using supernatants (18-h cultures) of a PSMδ-over-expressing <i>agr</i>-negative (lacking intrinsic PSM production) and corresponding control strains (incubation time, 6 h). pTX<i>psm</i>δ, pTX construct expressing PSMδ; pTX16, control plasmid. Strains were grown in TSB with 0.5% xylose and 12.5 µg/ml tetracycline. **, p<0.01, paired t-tests. Blue bars, PSMδ concentration in the culture filtrates relative to that in the 1457 wild-type (set to 100%).</p>