10.1371/journal.ppat.1001133.g004
Gordon Y. C. Cheung
Gordon
Y. C. Cheung
Kevin Rigby
Kevin
Rigby
Rong Wang
Rong
Wang
Shu Y. Queck
Shu
Y. Queck
Kevin R. Braughton
Kevin
R. Braughton
Adeline R. Whitney
Adeline
R. Whitney
Martin Teintze
Martin
Teintze
Frank R. DeLeo
Frank
R. DeLeo
Michael Otto
Michael
Otto
Neutrophil lysis by <i>S. epidermidis</i> PSM peptides and culture filtrates of PSMδ-expression strains.
Public Library of Science
2013
lysis
psm
peptides
filtrates
2013-02-20 23:01:30
Figure
https://plos.figshare.com/articles/figure/_Neutrophil_lysis_by_S_epidermidis_PSM_peptides_and_culture_filtrates_of_PSM_expression_strains_/496312
<p>(A) Neutrophil (PMN) lysis by synthetic, N-formylated PSM peptides at 10 µg/ml was determined by measuring release of LDH (incubation time, 1 h). PSMα3 (<i>S. aureus</i>) was used as a comparison at the same concentration. (B) Neutrophil lysis using supernatants (18-h cultures) of a PSMδ-over-expressing <i>agr</i>-negative (lacking intrinsic PSM production) and corresponding control strains (incubation time, 6 h). pTX<i>psm</i>δ, pTX construct expressing PSMδ; pTX16, control plasmid. Strains were grown in TSB with 0.5% xylose and 12.5 µg/ml tetracycline. **, p<0.01, paired t-tests. Blue bars, PSMδ concentration in the culture filtrates relative to that in the 1457 wild-type (set to 100%).</p>