%0 Figure %A Wang, Qian %A Xue, Huijun %A Li, Siqi %A Chen, Ying %A Tian, Xuelei %A Xu, Xin %A Xiao, Wei %A Fu, Yu Vincent %D 2017 %T PCR analysis of tag-HHT1 yeast strains and their subcellular localizations. %U https://plos.figshare.com/articles/figure/PCR_analysis_of_tag-_i_HHT1_i_yeast_strains_and_their_subcellular_localizations_/4958201 %R 10.1371/journal.pone.0176184.g003 %2 https://ndownloader.figshare.com/files/8345708 %K selectable markers %K HA %K target genes %K genomic loci %K RAD 5 %K protein tag %K UBC 13 %K epitope tags %K tag HHT 1 %K epitope tag %K study target proteins %K protein fusion tags %K yeast Saccharomyces cerevisiae %K chromosomal location %K counter-selection system %K chromosomal locus %K target gene %X

(A) HHT1-sfGFP strain. The genomic DNA PCR data shows in the left panel. Lane 1: DNA ladder marker. Lane 2: the original strain yRH182. Lane 3 and 4: the pop-in and pop-out strain. (B) The subcellular localizations of HHT1-sfGFP. The yRH182 (upper) is the wild-type yeast strains without modification; the tagged strains HHT1-sfGFP are shown at the bottom. The images were obtained under Plan-Apochromat 63×/1.40 oil (Zeiss 5 Live).

%I PLOS ONE