TY - DATA T1 - Dataset for: Cellular distribution and function of ion channels involved in transport processes in rat tracheal epithelium PY - 2017/07/17 AU - Anne Hahn AU - Johannes Faulhaber AU - Lalita Srisawang AU - Andreas Stortz AU - Johanna Jessica Salomon AU - Marcus A. Mall AU - Stephan Frings AU - Frank Möhrlen UR - https://wiley.figshare.com/articles/dataset/Dataset_for_Cellular_distribution_and_function_of_ion_channels_involved_in_transport_processes_in_rat_tracheal_epithelium/4929518 DO - 10.6084/m9.figshare.4929518.v1 L4 - https://ndownloader.figshare.com/files/8296742 KW - chloride secretion KW - tracheal epithelium KW - anoctamin1/TMEM16A KW - ion transport KW - Physiology KW - Systems Biology N2 - Transport of water and electrolytes in airway epithelia involves chloride-selective ion channels, which are controlled either by cytosolic Ca2+ or by cAMP. The contributions of the two pathways to chloride transport differ among vertebrate species. Because rats are becoming more important as animal model for cystic fibrosis, we have examined how Ca2+- dependent and cAMP- dependent Cl- secretion is organized in the rat tracheal epithelium. We examined the expression of the Ca2+-gated Cl- channel anoctamin 1 (ANO1), the cystic fibrosis transmembrane conductance regulator (CFTR) Cl- channel, the epithelial Na+ channel ENaC, and the water channel aquaporin 5 (AQP5) in rat tracheal epithelium. The contribution of ANO1 channels to nucleotide-stimulated Cl- secretion was determined using the channel blocker Ani9 in short-circuit current recordings obtained from primary cultures of rat tracheal epithelial cells in Ussing chambers. We found that ANO1, CFTR and AQP5 proteins were expressed in non-ciliated cells of the tracheal epithelium, whereas ENaC was expressed in ciliated cells. Among non-ciliated cells, ANO1 occurred together with CFTR and Muc5b and, in addition, in a different cell type without CFTR and Muc5b. Bioelectrical studies with the ANO1-blocker Ani9 indicated that ANO1 mediated the secretory response to the nucleotide uridine-5'-triphosphate. Our data demonstrate that, in rat tracheal epithelium, Cl- secretion and Na+ absorption are routed through different cell types, and that ANO1 channels form the molecular basis of Ca2+-dependent Cl- secretion in this tissue. These characteristic features of Cl--dependent secretion reveal similarities and distinct differences to secretory processes in the human airways. ER -