TY - DATA T1 - Peptidoglycan–polysaccharide (PG-PS)-induced activation of nuclear factor-κB (NF-κB) is inhibited in CD14 knockout spleen macrophages PY - 2011/12/31 AU - Xiangli Li AU - Blair U Bradford AU - Frederick Dalldorf AU - Sanna M Goyert AU - Stephen A Stimpson AU - Ronald G Thurman AU - Sergei S Makarov UR - https://figshare.com/articles/figure/Peptidoglycan_polysaccharide_PG_PS_induced_activation_of_nuclear_factor_B_NF_B_is_inhibited_in_CD14_knockout_spleen_macrophages/49157 DO - 10.6084/m9.figshare.49157.v1 L4 - https://ndownloader.figshare.com/files/48539 KW - peptidoglycanpolysaccharide KW - activation KW - nuclear KW - factorb KW - inhibited KW - knockout KW - spleen KW - macrophages N2 - Copyright information:Taken from "CD14 mediates the innate immune responses to arthritopathogenic peptidoglycan–polysaccharide complexes of Gram-positive bacterial cell walls"Arthritis Research & Therapy 2004;6(3):R273-R281.Published online 27 Apr 2004PMCID:PMC416450.Copyright © 2004 Li et al.; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL. Spleen macrophages were stimulated for 30 min with PG-PS at a concentration of 20 μg/ml. The left column shows immunostaining of the p65 (RelA) subunit of NF-κB, and the right column shows counterstaining of nuclei with Hoechst 33342. The rows show the following: top – unstimulated cells from wild-type mice; middle – PG-PS stimulated cells from wild-type mice; and bottom row – PG-PS-stimulated cells from CD14 knockout mice. Representative data from three independent experiments are shown. ER -