10.6084/m9.figshare.49150.v1 Judith van Holten Judith van Holten Kris Reedquist Kris Reedquist Pascale Sattonet-Roche Pascale Sattonet-Roche Tom JM Smeets Tom JM Smeets Christine Plater-Zyberk Christine Plater-Zyberk Margriet J Vervoordeldonk Margriet J Vervoordeldonk Paul P Tak Paul P Tak Representative histologic staining with safranin O–fast green (×100) of the ankle joint of mice with collagen-induced arthritis (CIA) mice after daily IFN-β therapy for 7 days figshare 2011 representative ifn daily arthritis collageninduced mice joint ankle green ofast safranin staining histologic therapy Molecular Biology 2011-12-31 04:37:40 Figure https://figshare.com/articles/figure/Representative_histologic_staining_with_safranin_O_fast_green_100_of_the_ankle_joint_of_mice_with_collagen_induced_arthritis_CIA_mice_after_daily_IFN_therapy_for_7_days/49150 <p><b>Copyright information:</b></p><p>Taken from "Treatment with recombinant interferon-β reduces inflammation and slows cartilage destruction in the collagen-induced arthritis model of rheumatoid arthritis"</p><p>Arthritis Research & Therapy 2004;6(3):R239-R249.</p><p>Published online 23 Mar 2004</p><p>PMCID:PMC416442.</p><p>Copyright © 2004 van Holten et al., licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.</p> (Upper panel) In CIA mice treated with saline as controls, hardly any safranin O staining was observed in the ankle joints. (Lower panel) In CIA mice treated daily with 2.5 μg IFN-β, significantly less loss of safranin O staining was observed, indicating inhibition of cartilage breakdown. Histologic analysis of cartilage in CIA in mice after 7 days of IFN-β therapy. Hind paw sections were stained with safranin O–fast green, which stains the cartilage proteoglycans. Sections were scored in a blinded manner on a 4-point scale as described in Materials and methods. Significantly less loss of safranin O staining was observed in the animals treated with IFN 2.5 μg than in controls (= 0.03), indicating inhibition of cartilage destruction. *Statistically significant difference.