Supplementary Material for: Differential Allelic Expression of HTR1B in Suicide Victims: Genetic and Epigenetic Effect of the Cis-Acting Variants
Bani-Fatemi A.
Howe A.
Zai C.
Kennedy J.L.
Vincent J.
Strauss J.
Wong A.
De Luca V.
10.6084/m9.figshare.4910612.v1
https://karger.figshare.com/articles/journal_contribution/Supplementary_Material_for_Differential_Allelic_Expression_of_HTR1B_in_Suicide_Victims_Genetic_and_Epigenetic_Effect_of_the_Cis-Acting_Variants/4910612
<p><b><i>Objectives:</i></b> In the present study, we tested the allelic
imbalance of the C861G single nucleotide polymorphism (SNP) of HTR1B in
the frontal cortex of suicide victims. <b><i>Methods:</i></b> The study
was conducted using 3 sets of samples. First, C861G allele-specific
mRNA levels in the frontal cortex were compared between suicide (<i>n</i> = 13) and nonsuicide controls (<i>n</i>
= 13) from the Stanley Medical Research postmortem brain collection.
Second, we tested common variants in the HTR1B promoter for linkage
disequilibrium (LD) with the C861G variant in an unrelated sample of
suicide attempters (SA; <i>n</i> = 38) and non-SA (NSA; <i>n</i> = 42).
Finally, we performed a family-based association study of the C861G and
promoter variants in 162 nuclear families using suicidal behavior
severity scores as phenotype. <b><i>Results:</i></b> We observed no alterations in the C/G expression ratio in suicide victims compared to nonsuicide controls (<i>p</i> = 0.370). When comparing the LD between the C861G and <i>cis</i>-acting SNPs, we did not find any differences in SA and NSA. There was no association between preferential transmission of <i>cis</i>-acting SNPs and suicidal behavior severity scores in both maternal and paternal meiosis. <b><i>Conclusions:</i></b>
We found several promoter variants in LD that may potentially influence
the allelic imbalance in the C861G variant. However, no evidence of
allelic imbalance nor parent-of-origin effects of the C861G variant was
observed in suicidal behavior. Further research is required to assess
this marker in larger cohorts.</p>
2017-04-26 14:49:00
Suicide
Bipolar disorder
HTR1B
Differential allelic expression
Parent-of-origin effects