10.6084/m9.figshare.4900607
Jennifer B. Glass
Jennifer B.
Glass
Si Chen
Si
Chen
Katherine S. Dawson
Katherine S.
Dawson
Damian R. Horton
Damian R.
Horton
Stefan Vogt
Stefan
Vogt
Ellery D. Ingall
Ellery D.
Ingall
Benjamin S. Twining
Benjamin S.
Twining
Victoria J. Orphan
Victoria J.
Orphan
Trace Metal Imaging of Sulfate-Reducing Bacteria and Methanogenic Archaea at Single-Cell Resolution by Synchrotron X-Ray Fluorescence Imaging
Taylor & Francis Group
2017
Metals
methanogen
methanol
sulfate reduction
synchrotron X-ray fluorescence
2017-05-19 14:43:20
Journal contribution
https://tandf.figshare.com/articles/journal_contribution/Trace_metal_imaging_of_sulfate-reducing_bacteria_and_methanogenic_archaea_at_single-cell_resolution_by_synchrotron_X-ray_fluorescence_imaging/4900607
<p>Metal cofactors are required for many enzymes in anaerobic microbial respiration. This study examined iron, cobalt, nickel, copper, and zinc in cellular and abiotic phases at the single-cell scale for a sulfate-reducing bacterium (<i>Desulfococcus multivorans</i>) and a methanogenic archaeon (<i>Methanosarcina acetivorans</i>) using synchrotron X-ray fluorescence microscopy. Relative abundances of cellular metals were also measured by inductively coupled plasma mass spectrometry. For both species, zinc and iron were consistently the most abundant cellular metals. <i>M. acetivorans</i> contained higher nickel and cobalt content than <i>D. multivorans</i>, likely due to elevated metal requirements for methylotrophic methanogenesis. Cocultures contained spheroid zinc sulfides and cobalt/copper sulfides.</p>