10.6084/m9.figshare.c.3747815_D4.v1
Saara Tegelberg
Saara
Tegelberg
Nikica Tomašić
Nikica
Tomašić
Jukka Kallijärvi
Jukka
Kallijärvi
Janne Purhonen
Janne
Purhonen
Eskil Elmér
Eskil
Elmér
Eva Lindberg
Eva
Lindberg
David Nord
David
Nord
Maria Soller
Maria
Soller
Nicole Lesko
Nicole
Lesko
Anna Wedell
Anna
Wedell
Helene Bruhn
Helene
Bruhn
Christoph Freyer
Christoph
Freyer
Henrik Stranneheim
Henrik
Stranneheim
Rolf Wibom
Rolf
Wibom
Inger Nennesmo
Inger
Nennesmo
Anna Wredenberg
Anna
Wredenberg
Erik Eklund
Erik
Eklund
Vineta Fellman
Vineta
Fellman
Additional file 4: Figure S3. of Respiratory chain complex III deficiency due to mutated BCS1L: a novel phenotype with encephalomyopathy, partially phenocopied in a Bcs1l mutant mouse model
Springer Nature
2017
Mitochondrial disorder
Respiratory chain
Respirometry
Assembly factors
Blue native gel electrophoresis
Encephalopathy
Hepatopathy
Microglia
Barrel cortex
2017-04-20 05:00:00
Journal contribution
https://springernature.figshare.com/articles/journal_contribution/Additional_file_4_Figure_S3_of_Respiratory_chain_complex_III_deficiency_due_to_mutated_BCS1L_a_novel_phenotype_with_encephalomyopathy_partially_phenocopied_in_a_Bcs1l_mutant_mouse_model/4897988
BNGE with immunblotting. The samples were run on two gels in quadruplicate. (A). The gel was stained with commassie blue after blotting to PVDF membrane to show that the loading was similar; the first (lanes 2-5) and second (lanes 7-10) loading of the samples with the ladder (lanes 1 and 6) are shown. The molecular weights of the ladder markers are indicated. (B). For the upper blot the CORE1 and RISP antibodies were used, for the second blot the BCS1L antibody and the combination of CI NDUFVI (to detect the subunit assembled at the final stage), CIV Va, and CII 30kD were used, respectively. The first blot was stripped and thereafter the antibodies against CIV COX and CI NDUFA9 were probed (remnants of the CORE1 and RISP bands can be seen). Despite weaker bands in the patient (lanes 1 and 6) the decrease in BCS1L and RISP is recognizable. (PDF 2082 kb)