%0 Figure %A Yaren, Ozlem %A Alto, Barry %A Gangodkar, Priyanka %A Ranade, Shatakshi %A Patil, Kunal %A Bradley, Kevin %A Yang, Zunyi %A Phadke, Nikhil %A Benner, Steven %D 2017 %T Additional file 4: Figure S3. of Point of sampling detection of Zika virus within a multiplexed kit capable of detecting dengue and chikungunya %U https://springernature.figshare.com/articles/figure/Additional_file_4_Figure_S3_of_Point_of_sampling_detection_of_Zika_virus_within_a_multiplexed_kit_capable_of_detecting_dengue_and_chikungunya/4897793 %R 10.6084/m9.figshare.c.3747752_D4.v1 %2 https://ndownloader.figshare.com/files/8232182 %K Point-of-care diagnostics %K Multiplexed isothermal amplification %K Zika detection %K Fluorescence read-out %K Sample preparation %K Mosquito surveillance %K Virus detection %X Limit of detection for 1-plex chikungunya and dengue-1 RT-LAMP experiments. Substrates for this experiment were extracted viral RNA from Vero cell cultures. (A) Varying titers of chikungunya viral RNAs (~189 to 18 copies) were included in RT-LAMP reagents and run real-time using Light cycler (channel 523-568). For chikungunya detection, 80 nM of HEX-labeled probes were used, and about 38 copies of chikungunya viral RNA could be detected in less than 30 min. (B) Varying titers of dengue-1 viral RNAs (~2.44 to 0.12 pfu equivalent RNA copies) were included in RT-LAMP reagents and run real-time using Light cycler (channel 558-610). For dengue-1 detection, 80 nM of TAMRA-labeled probes were used, and about 1.22 pfu equivalent copies of dengue-1a viral RNA could be detected within 35 min (JPEG 58 kb) %I figshare