Walter, T. Crews, Fulton Additional file 8: Figure S8. of Microglial depletion alters the brain neuroimmune response to acute binge ethanol withdrawal Continuous ethanol treatment does not increase BV2 TNFα or Ccl2 expression. Microglia-like BV2 cells were treated with either ethanol (85 mM) or PBS. A) For ethanol-treated cells, ethanol was vaporized into the incubator to keep media ethanol concentrations constant. After 24 h of continuous ethanol exposure, BV2 mRNA was isolated and B) TNFα and C) Ccl2 gene expression were measured. Note that continuous ethanol exposure does not increase BV2 pro-inflammatory cytokine expression at 24 h. Data are represented as mean ± SEM. *p < 0.05, Student’s t test, n = 6/group (TIF 94 kb) Microglia;Binge;Ethanol;Withdrawal;Neuroimmune;Pro-inflammatory;Anti-inflammatory 2017-04-20
    https://springernature.figshare.com/articles/figure/Additional_file_8_Figure_S8_of_Microglial_depletion_alters_the_brain_neuroimmune_response_to_acute_binge_ethanol_withdrawal/4897355
10.6084/m9.figshare.c.3747563_D11.v1