Improving Sample Distribution Homogeneity in Three-Dimensional
Microfluidic Paper-Based Analytical Devices by Rational Device Design
Giorgio
Gianini Morbioli
Thiago Mazzu-Nascimento
Luis Aparecido Milan
Amanda M. Stockton
Emanuel Carrilho
10.1021/acs.analchem.6b04953.s003
https://acs.figshare.com/articles/media/Improving_Sample_Distribution_Homogeneity_in_Three-Dimensional_Microfluidic_Paper-Based_Analytical_Devices_by_Rational_Device_Design/4891769
Paper-based
devices are a portable, user-friendly, and affordable
technology that is one of the best analytical tools for inexpensive
diagnostic devices. Three-dimensional microfluidic paper-based analytical
devices (3D-μPADs) are an evolution of single layer devices
and they permit effective sample dispersion, individual layer treatment,
and multiplex analytical assays. Here, we present the rational design
of a wax-printed 3D-μPAD that enables more homogeneous permeation
of fluids along the cellulose matrix than other existing designs in
the literature. Moreover, we show the importance of the rational design
of channels on these devices using glucose oxidase, peroxidase, and
2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS)
reactions. We present an alternative method for layer stacking using
a magnetic apparatus, which facilitates fluidic dispersion and improves
the reproducibility of tests performed on 3D-μPADs. We also
provide the optimized designs for printing, facilitating further studies
using 3D-μPADs.
2017-04-12 00:00:00
wax-printed 3 D -μPAD
ABTS
optimized designs
layer devices
glucose oxidase
Three-Dimensional Microfluidic Paper-Based Analytical Devices
alternative method
Rational Device Design Paper-based devices
cellulose matrix
Sample Distribution Homogeneity
3 D -μPADs
sample dispersion
Three-dimensional microfluidic paper-based
fluidic dispersion
layer treatment