**A.** GFP-Atg8-expressing cells were infected for 5 h with mCherry-expressing *M*. *marinum* wt and treated or not with AR-12 at 2.5 μM for 2 additional hours. Representative maximum projections of live imaging are shown. White arrowheads point to GFP-Atg8 recruitment to MCV. Scale bars, 10 μm; **B.** Quantification of the percentage of infected cells with GFP-Atg8^{+} bacteria at 7 hpi. Means and standard deviations from six (mock) and three (AR-12) independent replicates are represented and an unpaired *t* test showed no statistical significance (ns, *p* > 0.05). 688 and 297 infected cells were counted for the mock and the AR-12 treatments, respectively; **C.** Classification of types of GFP-recruitment for infected mock (258 cells) and AR-12 (141 cells) treated. Means and standard deviations from six (mock) and three (AR-12) independent replicates are represented. Unpaired *t* test (***p* ≤ 0.01; ****p* ≤ 0.001); **D.** Cells infected with *lux*-expressing *M*. *marinum* wt bacteria were treated or not with AR-12 at 2.5 μM. Intracellular bacteria growth [relative luminescence units (RLU)] is represented as the mean and standard deviation from triplicates. Statistical differences were calculated with a Bonferroni post hoc test after two-way ANOVA (**p* ≤ 0.05).