TY - DATA T1 - The accumulation of SUMO conjugates is dependent on virus reactivation. PY - 2017/04/17 AU - Jinlin Li AU - Simone Callegari AU - Maria G. Masucci UR - https://plos.figshare.com/articles/figure/The_accumulation_of_SUMO_conjugates_is_dependent_on_virus_reactivation_/4883525 DO - 10.1371/journal.ppat.1006338.g002 L4 - https://ndownloader.figshare.com/files/8347631 KW - SUMO-targeted ubiquitin ligase RNF 4. KW - virus Post-translational modification KW - SUMO conjugation machinery KW - Small Ubiquitin-like Modifier KW - inducible miR-BHRF 1-1 sponge KW - Epstein-Barr virus miR-BHRF 1-1 targets RNF 4 KW - EBV miR-BHRF 1-1 KW - miR-BHRF 1-1. Reconstitution KW - RNF 4 protein expression KW - RNF 4 KW - virus cycle N2 - (A) Western blots of untreated and anti-IgG treated EBV negative Akata and EBV positive Akata-Bx1 and TPA/Bu treated EBV negative AGS and EBV positive AGS-Bx1 were probed with antibodies to SUMO2/3, BZLF1 and β-actin. Induction of the productive virus cycle was accompanied by accumulation of poly-SUMOylated proteins in the EBV positive cell lines. (B) The intensities of the SUMO2/3 reactive bands were quantified by densitometry. The average increase relative to untreated controls in two independent experiments is shown in the figure. ER -