Supplementary Material for: The Inhibitory Effect of Eplerenone on Cell Proliferation in the Contralateral Kidneys of Rats with Unilateral Ureteral Obstruction
Wang C.
Wang Z.
Liang L.
Wang X.
Ma X.
Liu B.
He J.
Shimosawa T.
Xu Q.
10.6084/m9.figshare.4872668.v1
https://karger.figshare.com/articles/dataset/Supplementary_Material_for_The_Inhibitory_Effect_of_Eplerenone_on_Cell_Proliferation_in_the_Contralateral_Kidneys_of_Rats_with_Unilateral_Ureteral_Obstruction/4872668
<p><b><i>Background:</i></b> The unilateral ureteral obstruction (UUO)
model not only induces renal interstitial fibrosis in the obstructed
kidney but also induces injury in the contralateral kidney. We
hypothesized that activation of the mineralocorticoid receptor (MR) may
induce fibrosis in the early stage of UUO. <b><i>Methods:</i></b> Thirty
male Sprague-Dawley rats weighting 200 ± 10 g were used in this study
and randomly divided into 3 groups: a UUO group, a UUO and eplerenone
group, and a sham group. The contralateral kidney and plasma were
harvested for further study 10 days after surgery. <b><i>Results:</i></b>
The level of plasma aldosterone (869.95 ± 55.851 pg/mL) was
significantly higher in the UUO group than that in the sham group
(478.581 ± 36.186 pg/mL vs. UUO, <i>p</i> < 0.05). The infiltrated
inflammatory cells (F4/80) and deposited collagens were increased
significantly in the contralateral kidneys in the UUO group compared to
those in the sham group, which were decreased by eplerenone. However,
proliferating cell nuclear antigen was increased 2.47 times in the UUO
group compared to the sham group in the contralateral kidney (<i>p</i>
< 0.01), and those changes are attenuated by eplerenone. The
expression of SGK-1 protein and mRNA was upregulated in the
contralateral kidney in the UUO group, which is suppressed by eplerenone
treatment. NF-κB pathway effecters were also changed markedly in the
contralateral kidney in the UUO group and partly reversed by eplerenone.
<b><i>Conclusion:</i></b> Aldosterone induces inflammatory cell
proliferation via the MR/SGK-1 and NF-κB pathways and eventually leads
to fibrosis in the contralateral kidney.</p>
2017-04-13 06:48:44
Aldosterone
Mineralocorticoid receptor
Proliferation
Inflammation
Fibrosis
Contralateral kidney