%0 Figure %A Tian, Juan %A Zhang, Xueyan %A Liang, Benguo %A Li, Shanwei %A Wu, Zhixia %A Wang, Qianhua %A Leng, Chunxu %A Dong, Jiangli %A Wang, Tao %D 2013 %T Southern blot analysis of three T3 transgenic cotton lines. %U https://plos.figshare.com/articles/figure/_Southern_blot_analysis_of_three_T_3_transgenic_cotton_lines_/485246 %R 10.1371/journal.pone.0014218.g003 %2 https://ndownloader.figshare.com/files/814882 %K blot %K transgenic %X

Southern blot hybridization analysis of genomic DNA extracted from leaves of three transgenic cotton plants and non-transformed negative control plants. (a) DNA from transgenic cotton plants and non-transformed negative control plants was digested with EcoRI and hybridized with a DIG-labeled p35 probe (870 bp); (b) DNA from three transgenic cotton plants and non-transformed negative control plants was digested with EcoRI and hybridized with a DIG-labeled op-iap probe (583 bp); Lane 1 the negative control plant; lanes 2, 3, 4 the three transgenics lines (KB1, KB2, KB3); lane 5: positive control [purified PCR products of the p35 (870 bp) and op-iap (583 bp) coding region]; lane M molecular mass marker is GeneRuler TM 1 kb DNA ladder (MBI Fermentas, Maryland, USA).

%I PLOS ONE