TY - DATA T1 - Analysis of SNP rs2104772 by High-Resolution Melt analysis (HRM). PY - 2017/04/06 AU - Paola Valdivieso AU - Marco Toigo AU - Hans Hoppeler AU - Martin Flück UR - https://plos.figshare.com/articles/figure/Analysis_of_SNP_rs2104772_by_High-Resolution_Melt_analysis_HRM_/4824904 DO - 10.1371/journal.pone.0174864.g002 L4 - https://ndownloader.figshare.com/files/8012980 KW - ANOVA KW - endurance exercise KW - density KW - Tenascin-C protein expression KW - 8 angiogenesis-associated transcripts KW - factor KW - pro-angiogenic extracellular matrix protein tenascin-C KW - capillary KW - SNP KW - acid codon 1677 KW - Genotype KW - blood vessel-related expression KW - tenascin-C gene polymorphism rs 2104772 KW - influences exercise-induced angiogenesis Background Mechanical stress KW - Results Endurance training KW - homozygosity KW - carrier KW - vastus lateralis muscle KW - VEGF KW - 30- min bouts KW - volume KW - homozygote N2 - A-C) Line graph showing the detected or derived fluorescence intensity for the analyzed SNP (i.e. melting curves). The displayed examples included measurements for A/A homozygotes (red line, n = 5)) and T/T homozygotes (blue line, n = 3) relative to the heterozygote A/T (green line, n = 12). Every sample was analyzed in duplicate. A) Raw data of the pre-melt, melt, and post-melt regions. B) Normalized data derived from the raw data plots. C) Melting curves derived after normalization versus the A/A genotype. D-F) Sequence analysis of the three identified genotypes in chromatograms presenting the forward sequence. Arrows link the single-nucleotide polymorphism (SNP) position 44513. The presence of 'W' in A/T genotype denotes heterozygosis for the SNP where a double-peak is present at position 44513 for both sequenced alleles (arrow, nucleotides A and T simultaneously). ER -