%0 Figure %A Valdivieso, Paola %A Toigo, Marco %A Hoppeler, Hans %A Flück, Martin %D 2017 %T Analysis of SNP rs2104772 by High-Resolution Melt analysis (HRM). %U https://plos.figshare.com/articles/figure/Analysis_of_SNP_rs2104772_by_High-Resolution_Melt_analysis_HRM_/4824904 %R 10.1371/journal.pone.0174864.g002 %2 https://ndownloader.figshare.com/files/8012980 %K ANOVA %K endurance exercise %K density %K Tenascin-C protein expression %K 8 angiogenesis-associated transcripts %K factor %K pro-angiogenic extracellular matrix protein tenascin-C %K capillary %K SNP %K acid codon 1677 %K Genotype %K blood vessel-related expression %K tenascin-C gene polymorphism rs 2104772 %K influences exercise-induced angiogenesis Background Mechanical stress %K Results Endurance training %K homozygosity %K carrier %K vastus lateralis muscle %K VEGF %K 30- min bouts %K volume %K homozygote %X

A-C) Line graph showing the detected or derived fluorescence intensity for the analyzed SNP (i.e. melting curves). The displayed examples included measurements for A/A homozygotes (red line, n = 5)) and T/T homozygotes (blue line, n = 3) relative to the heterozygote A/T (green line, n = 12). Every sample was analyzed in duplicate. A) Raw data of the pre-melt, melt, and post-melt regions. B) Normalized data derived from the raw data plots. C) Melting curves derived after normalization versus the A/A genotype. D-F) Sequence analysis of the three identified genotypes in chromatograms presenting the forward sequence. Arrows link the single-nucleotide polymorphism (SNP) position 44513. The presence of 'W' in A/T genotype denotes heterozygosis for the SNP where a double-peak is present at position 44513 for both sequenced alleles (arrow, nucleotides A and T simultaneously).

%I PLOS ONE