10.6084/m9.figshare.c.3730981_D3.v1 Akira Yamauchi Akira Yamauchi Masahiro Yamamura Masahiro Yamamura Naoki Katase Naoki Katase Masumi Itadani Masumi Itadani Naoko Okada Naoko Okada Kayoko Kobiki Kayoko Kobiki Masafumi Nakamura Masafumi Nakamura Yoshiyuki Yamaguchi Yoshiyuki Yamaguchi Futoshi Kuribayashi Futoshi Kuribayashi Additional file 7: of Evaluation of pancreatic cancer cell migration with multiple parameters in vitro by using an optical real-time cell mobility assay device Springer Nature 2017 Migration Chemotaxis Lipid mediator Inhibitor TAXIScan Metastasis 2017-03-31 05:00:00 Figure https://springernature.figshare.com/articles/figure/Additional_file_7_of_Evaluation_of_pancreatic_cancer_cell_migration_with_multiple_parameters_in_vitro_by_using_an_optical_real-time_cell_mobility_assay_device/4806058 The modified Boyden chamber assay. A) Schematic diagram (sagittal section) of one well of the modified Boyden chamber assay (Transwell). Cells in the chemotaxis buffer are located in the upper chamber and the chemoattractant the chemotaxis buffer is added to the lower chamber. B) Schematic diagram of the membrane part of the modified Boyden chamber. The membrane separates the upper and the lower chamber. The matrix is coated on the lower side of the membrane. C) Photographs of the lower side of the membrane after the assay. Cells are stained with the staining solution accompanied with the assay kit. Magnification: 400×. (TIFF 98113 kb)