10.6084/m9.figshare.c.3730981_D3.v1
Akira Yamauchi
Akira
Yamauchi
Masahiro Yamamura
Masahiro
Yamamura
Naoki Katase
Naoki
Katase
Masumi Itadani
Masumi
Itadani
Naoko Okada
Naoko
Okada
Kayoko Kobiki
Kayoko
Kobiki
Masafumi Nakamura
Masafumi
Nakamura
Yoshiyuki Yamaguchi
Yoshiyuki
Yamaguchi
Futoshi Kuribayashi
Futoshi
Kuribayashi
Additional file 7: of Evaluation of pancreatic cancer cell migration with multiple parameters in vitro by using an optical real-time cell mobility assay device
Springer Nature
2017
Migration
Chemotaxis
Lipid mediator
Inhibitor
TAXIScan
Metastasis
2017-03-31 05:00:00
Figure
https://springernature.figshare.com/articles/figure/Additional_file_7_of_Evaluation_of_pancreatic_cancer_cell_migration_with_multiple_parameters_in_vitro_by_using_an_optical_real-time_cell_mobility_assay_device/4806058
The modified Boyden chamber assay. A) Schematic diagram (sagittal section) of one well of the modified Boyden chamber assay (Transwell). Cells in the chemotaxis buffer are located in the upper chamber and the chemoattractant the chemotaxis buffer is added to the lower chamber. B) Schematic diagram of the membrane part of the modified Boyden chamber. The membrane separates the upper and the lower chamber. The matrix is coated on the lower side of the membrane. C) Photographs of the lower side of the membrane after the assay. Cells are stained with the staining solution accompanied with the assay kit. Magnification: 400Ă. (TIFF 98113Â kb)