10.6084/m9.figshare.c.3728662_D1.v1
Fiona Guan
Fiona
Guan
Charles Bailey
Charles
Bailey
Cynthia Metierre
Cynthia
Metierre
Patrick O’Young
Patrick
O’Young
Dadi Gao
Dadi
Gao
Teh Khoo
Teh
Khoo
Jeff Holst
Jeff
Holst
John Rasko
John
Rasko
Additional file 10: Figure S5. of The antiproliferative ELF2 isoform, ELF2B, induces apoptosis in vitro and perturbs early lymphocytic development in vivo
Springer Nature
2017
ELF2
ELF2A
ELF2B
Isoform
DNA binding
Ets domain
Transcription factor
Dominant negative
Antiproliferative
Apoptosis
Lymphoid development
Tumour suppressor
2017-03-28 05:00:00
Journal contribution
https://springernature.figshare.com/articles/journal_contribution/Additional_file_10_Figure_S5_of_The_antiproliferative_ELF2_isoform_ELF2B_induces_apoptosis_in_vitro_and_perturbs_early_lymphocytic_development_in_vivo/4796872
Reconstitution efficiency in ELF2+ retrogenic mice. A) Murine stem cell virus-based (MSCV) retroviral vector (pMIG) used for expressing HA-tagged Elf2 isoforms; primer sequences used for detecting specific isoform expression are indicated (arrowheads); a common 5’ primer within the HA-tag and 3’ primer able to detect all Elf2 isoforms were used. B) RT-qPCR of ectopic Elf2a isoform expression in the spleens of retrogenic mice after 3 months reconstitution. Analysis of GFP expression after 4 weeks in peripheral blood mononuclear cells: total C); T cell population D); B cell population E); and granulocytes F). Reconstitution efficiency in the haemopoietic compartment after 3 months. Data represents the mean ± SEM of 3 experiments each performed with 4–5 mice per experimental arm. Statistical analysis performed using Student’s t test (ns, not significant; *, p < 0.05; **, p < 0.01) (PDF 1218 kb)