10.6084/m9.figshare.c.3728662_D1.v1 Fiona Guan Fiona Guan Charles Bailey Charles Bailey Cynthia Metierre Cynthia Metierre Patrick O’Young Patrick O’Young Dadi Gao Dadi Gao Teh Khoo Teh Khoo Jeff Holst Jeff Holst John Rasko John Rasko Additional file 10: Figure S5. of The antiproliferative ELF2 isoform, ELF2B, induces apoptosis in vitro and perturbs early lymphocytic development in vivo Springer Nature 2017 ELF2 ELF2A ELF2B Isoform DNA binding Ets domain Transcription factor Dominant negative Antiproliferative Apoptosis Lymphoid development Tumour suppressor 2017-03-28 05:00:00 Journal contribution https://springernature.figshare.com/articles/journal_contribution/Additional_file_10_Figure_S5_of_The_antiproliferative_ELF2_isoform_ELF2B_induces_apoptosis_in_vitro_and_perturbs_early_lymphocytic_development_in_vivo/4796872 Reconstitution efficiency in ELF2+ retrogenic mice. A) Murine stem cell virus-based (MSCV) retroviral vector (pMIG) used for expressing HA-tagged Elf2 isoforms; primer sequences used for detecting specific isoform expression are indicated (arrowheads); a common 5’ primer within the HA-tag and 3’ primer able to detect all Elf2 isoforms were used. B) RT-qPCR of ectopic Elf2a isoform expression in the spleens of retrogenic mice after 3 months reconstitution. Analysis of GFP expression after 4 weeks in peripheral blood mononuclear cells: total C); T cell population D); B cell population E); and granulocytes F). Reconstitution efficiency in the haemopoietic compartment after 3 months. Data represents the mean ± SEM of 3 experiments each performed with 4–5 mice per experimental arm. Statistical analysis performed using Student’s t test (ns, not significant; *, p < 0.05; **, p < 0.01) (PDF 1218 kb)