10.6084/m9.figshare.4793359
Yongbiao Zheng
Yongbiao
Zheng
Li Xu
Li
Xu
Liufeng Wei
Liufeng
Wei
Mianmian Shi
Mianmian
Shi
Jiyan Zhang
Jiyan
Zhang
Jianzhong Huang
Jianzhong
Huang
A Sphingosine-type cerebroside in <i>Clavicorona pyxidata</i> induce fruit body formation
Taylor & Francis Group
2017
Clavicorona pyxidata
cerebroside
fruiting body formation
2017-03-28 08:57:54
Journal contribution
https://tandf.figshare.com/articles/journal_contribution/A_Sphingosine-type_cerebroside_in_i_Clavicorona_pyxidata_i_induce_fruit_body_formation/4793359
<p><i>Clavicorona pyxidata</i> is a wild edible and medicinal mushroom that is rich in bioactive natural products and has thus been extensively used as traditional medicine in China. The present study has determined that the organic crude extract prepared from a fermented culture of <i>C. pyxidata</i> imparted auto-inhibitory effects on mycelial growth and then induced the formation of fruiting bodies. By monitoring bioactivity, one compound was isolated via successive chromatography over silica gel, Sephadex LH-20, and Cl8-reversed phase silica gel and was identified as a known sphingosine-type cerebroside by nuclear magnetic resonance (NMR) and physicochemical data, namely, (4E, 8E)-N-D-2′-hydroxypalmitoyl-1-O-β-D-glucopyranosyl-9-methyl-4,8-sphingadienine. The application of this cerebroside at a concentration of 200 μg/disc paper resulted in the inhibition of aerial hyphal growth of <i>C. pyxidata.</i> The findings of the present study indicated that this <i>C. pyxidata</i> cerebroside is a fruiting body-inducing substance (FIS).</p>