TY - DATA T1 - Supplementary Material for: Cytosine Methylation of an Ancient Satellite Family in the Wild Beet Beta procumbens PY - 2017/03/13 AU - Schmidt M. AU - Hense S. AU - Minoche A.E. AU - Dohm J.C. AU - Himmelbauer H. AU - Schmidt T. AU - Zakrzewski F. UR - https://karger.figshare.com/articles/dataset/Supplementary_Material_for_Cytosine_Methylation_of_an_Ancient_Satellite_Family_in_the_Wild_Beet_Beta_procumbens/4747792 DO - 10.6084/m9.figshare.4747792.v1 L4 - https://ndownloader.figshare.com/files/7784683 L4 - https://ndownloader.figshare.com/files/7784686 L4 - https://ndownloader.figshare.com/files/7784689 L4 - https://ndownloader.figshare.com/files/7784692 L4 - https://ndownloader.figshare.com/files/7784695 L4 - https://ndownloader.figshare.com/files/7784698 L4 - https://ndownloader.figshare.com/files/7784701 L4 - https://ndownloader.figshare.com/files/7784704 L4 - https://ndownloader.figshare.com/files/7784707 L4 - https://ndownloader.figshare.com/files/7784710 KW - Beta procumbens KW - Bisulfite sequencing KW - DNA methylation KW - Immunostaining KW - Satellite DNA KW - Sugar beet N2 - DNA methylation is an essential epigenetic feature for the regulation and maintenance of heterochromatin. Satellite DNA is a repetitive sequence component that often occurs in large arrays in heterochromatin of subtelomeric, intercalary and centromeric regions. Knowledge about the methylation status of satellite DNA is important for understanding the role of repetitive DNA in heterochromatization. In this study, we investigated the cytosine methylation of the ancient satellite family pEV in the wild beet Beta procumbens. The pEV satellite is widespread in species-specific pEV subfamilies in the genus Beta and most likely originated before the radiation of the Betoideae and Chenopodioideae. In B. procumbens, the pEV subfamily occurs abundantly and spans intercalary and centromeric regions. To uncover its cytosine methylation, we performed chromosome-wide immunostaining and bisulfite sequencing of pEV satellite repeats. We found that CG and CHG sites are highly methylated while CHH sites show only low levels of methylation. As a consequence of the low frequency of CG and CHG sites and the preferential occurrence of most cytosines in the CHH motif in pEV monomers, this satellite family displays only low levels of total cytosine methylation. ER -