10.1371/journal.pone.0173628.g003 Asa Okuda-Tanino Asa Okuda-Tanino Daiki Sugawara Daiki Sugawara Takumi Tashiro Takumi Tashiro Masaya Iwashita Masaya Iwashita Yutaro Obara Yutaro Obara Takahiro Moriya Takahiro Moriya Chisato Tsushima Chisato Tsushima Daisuke Saigusa Daisuke Saigusa Yoshihisa Tomioka Yoshihisa Tomioka Kuniaki Ishii Kuniaki Ishii Norimichi Nakahata Norimichi Nakahata Scanning electron microscopy images of platelets stimulated by collagen in the presence of licochalcones. Public Library of Science 2017 COX platelet aggregation collagen-induced platelet aggregation licochalcone arachidonic acid-induced platelet aggregation Glycyrrhiza inflata ADP-induced platelet aggregation 2 receptor agonist enzyme reaction kinetics rabbit platelet aggregation arachidonic acid attenuated collagen-induced aggregation prostaglandin E 2 synthesis 2017-03-10 18:44:20 Figure https://plos.figshare.com/articles/figure/Scanning_electron_microscopy_images_of_platelets_stimulated_by_collagen_in_the_presence_of_licochalcones_/4745452 <p>Platelets were incubated with collagen (3 μg/ml) for 3 min in the presence or absence of licochalcones (100 μM), then fixed overnight with 1% glutaraldehyde. The samples were washed twice for 5 min with PBS. The fixed platelets were dehydrated with ethanol and t-butyl alcohol, and after the samples were freeze-dried and coated with Au/Pd, they were observed under a scanning electron microscope. <b>(A)</b> Unstimulated platelets, <b>(B)</b> collagen (3 μg/ml), <b>(C)</b> licochalcone A (100 μM) +collagen, <b>(D)</b> licochalcone C (100 μM) +collagen and <b>(E)</b> licochalcone D (100 μM) +collagen. (Magnification: 7000×, bar = 5 μm).</p>