10.1371/journal.pone.0173628.g003
Asa Okuda-Tanino
Asa
Okuda-Tanino
Daiki Sugawara
Daiki
Sugawara
Takumi Tashiro
Takumi
Tashiro
Masaya Iwashita
Masaya
Iwashita
Yutaro Obara
Yutaro
Obara
Takahiro Moriya
Takahiro
Moriya
Chisato Tsushima
Chisato
Tsushima
Daisuke Saigusa
Daisuke
Saigusa
Yoshihisa Tomioka
Yoshihisa
Tomioka
Kuniaki Ishii
Kuniaki
Ishii
Norimichi Nakahata
Norimichi
Nakahata
Scanning electron microscopy images of platelets stimulated by collagen in the presence of licochalcones.
Public Library of Science
2017
COX
platelet aggregation
collagen-induced platelet aggregation
licochalcone
arachidonic acid-induced platelet aggregation
Glycyrrhiza inflata
ADP-induced platelet aggregation
2 receptor agonist
enzyme reaction kinetics
rabbit platelet aggregation
arachidonic acid
attenuated collagen-induced aggregation
prostaglandin E 2 synthesis
2017-03-10 18:44:20
Figure
https://plos.figshare.com/articles/figure/Scanning_electron_microscopy_images_of_platelets_stimulated_by_collagen_in_the_presence_of_licochalcones_/4745452
<p>Platelets were incubated with collagen (3 μg/ml) for 3 min in the presence or absence of licochalcones (100 μM), then fixed overnight with 1% glutaraldehyde. The samples were washed twice for 5 min with PBS. The fixed platelets were dehydrated with ethanol and t-butyl alcohol, and after the samples were freeze-dried and coated with Au/Pd, they were observed under a scanning electron microscope. <b>(A)</b> Unstimulated platelets, <b>(B)</b> collagen (3 μg/ml), <b>(C)</b> licochalcone A (100 μM) +collagen, <b>(D)</b> licochalcone C (100 μM) +collagen and <b>(E)</b> licochalcone D (100 μM) +collagen. (Magnification: 7000×, bar = 5 μm).</p>