Community
Assembly and Ecology of Activated Sludge
under Photosynthetic Feast–Famine Conditions
Ben O. Oyserman
Joseph M. Martirano
Spenser Wipperfurth
Brian R. Owen
Daniel R. Noguera
Katherine D. McMahon
10.1021/acs.est.6b03976.s011
https://acs.figshare.com/articles/dataset/Community_Assembly_and_Ecology_of_Activated_Sludge_under_Photosynthetic_Feast_Famine_Conditions/4743451
Here, we demonstrate that photosynthetic
oxygen production under
light–dark and feast–famine cycles with no mechanical
aeration and negligible oxygen diffusion is able to maintain phosphorus
cycling activity associated with the enrichment of polyphosphate accumulating
organisms (PAOs). We investigate the ecology of this novel system
by conducting a time series analysis of prokaryotic and eukaryotic
biodiversity using the V3–V4 and V4 regions of the 16S and
18S rRNA gene sequences, respectively. In the Eukaryotic community,
the initial dominant alga observed was <i>Desmodesmus</i>. During operation, the algal community became a more diverse consortium
of <i>Desmodesmus</i>, <i>Parachlorella</i>, <i>Characiopodium</i>, and <i>Bacillariophytina</i>.
In the Prokaryotic community, there was an initial enrichment of the
PAO <i>Candidatus</i> Accumulibacter phosphatis (Accumulibacter)
Acc-SG2, and the dominant ammonia-oxidizing organism was <i>Nitrosomonas
oligotropha</i>; however, these populations decreased in relative
abundance, becoming dominated by Accumulibacter Acc-SG3 and <i>Nitrosomonas ureae</i>. Furthermore, functional guilds that
were not abundant initially became enriched including the putative <i>Cyanobacterial</i> PAOs <i>Obscuribacterales</i> and <i>Leptolyngbya</i> and the H<sub>2</sub>-oxidizing denitrifying
autotroph <i>Sulfuritalea</i>. After a month of operation,
the most-abundant prokaryote belonged to an uncharacterized clade
of <i>Chlorobi</i> classified as <i>Chlorobiales</i>;SJA-28 Clade III, the first reported enrichment of this lineage.
This experiment represents the first investigation into the ecological
interactions and community assembly during photosynthetic feast–famine
conditions. Our findings suggest that photosynthesis may provide sufficient
oxygen to drive polyphosphate cycling.
2017-02-27 00:00:00
V 4 regions
Prokaryotic community
eukaryotic biodiversity
time series analysis
uncharacterized clade
algal community
Eukaryotic community
oxygen diffusion
Accumulibacter Acc-SG 3
community Assembly
18 S rRNA gene sequences
Nitrosomonas ureae
Nitrosomonas oligotropha
Desmodesmu
phosphorus cycling activity
oxidizing denitrifying autotroph Sulfuritalea
H 2
Activated Sludge
photosynthetic oxygen production
PAO Candidatus Accumulibacter phosphatis
community assembly
ammonia-oxidizing organism
drive polyphosphate cycling
Cyanobacterial PAOs Obscuribacterales
novel system
SJA -28 Clade III
most-abundant prokaryote
16 S
enrichment