10.6084/m9.figshare.4733254.v1
Mohr C.
Mohr
C.
Kleiner S.
Kleiner
S.
Blanchette M.
Blanchette
M.
Pyrowolakis G.
Pyrowolakis
G.
Hartmann B.
Hartmann
B.
Supplementary Material for: Sex-Specific Transcript Diversity in the Fly Head Is Established during Pupal Stages and Adulthood and Is Largely Independent of the Mating Process and the Germline
Karger Publishers
2017
Alternative splicing
Neuronal development
Precursor mRNA
Drosophila
Sex differences
2017-03-08 13:32:17
Dataset
https://karger.figshare.com/articles/dataset/Supplementary_Material_for_Sex-Specific_Transcript_Diversity_in_the_Fly_Head_Is_Established_during_Pupal_Stages_and_Adulthood_and_Is_Largely_Independent_of_the_Mating_Process_and_the_Germline/4733254
<p>Alternative splicing (AS), the process which generates multiple RNA
and protein isoforms from a single pre-mRNA, greatly contributes to
transcript diversity and compensates for the fact that the gene number
does not scale with organismal complexity. A number of genomic
approaches have established that the extent of AS is much higher than
previously expected, raising questions on its spatio-temporal regulation
and function. In the present study, we address AS in the context of
sex-specific neuronal development in the model <i>Drosophila melanogaster</i>. We report that at least 47 genes display sex-specific AS in the adult fly head. Unlike targets of the classical <i>Sex lethal</i>-dependent
sex determination cascade, sex-specific isoforms of the vast majority
of these genes are not present during larval development but start
accumulating during metamorphosis or later, indicating the existence of
novel mechanisms in the induction of sex-specific AS. We also
established that sex-specific AS in the adult fly head is largely
independent of the germline or the mating process. Finally, we
investigated the role of sex-specific AS of the sulfotransferase <i>Tango13</i>
pre-mRNA and provide first evidence that differential expression of
certain isoforms of this protein significantly affects courtship and
mating behavior in male flies.</p>